The contribution of two phosphorylated surface modifications on the pathogenesis of Campylobacter upsaliensis

• Author / Creator

  Crowley, Shauna M

• Campylobacter upsaliensis is a human pathogen most commonly associated with
  self-limiting gastroenteritis. Despite extensive epidemiological data indicating C.
  upsaliensis as an emerging pathogen, few studies have examined possible
  mechanisms of its virulence. The surface of C. upsaliensis is comprised of
  capsular polysaccharides (CPS) and lipooligosaccharides (LOS), which are likely
  involved in bacterial interactions with its host and environment. In this study, we
  demonstrated that the LOS of C. upsaliensis RM3195 is modified with
  phosphocholine (PCho) and its CPS is decorated with phosphoramidate (MeOPN)
  residues. These phosphorylated moieties are involved in host cell invasion
  potentially mediated through the platelet activating factor receptor. Also, PCho
  and MeOPN conversely affect bacterial survival in human serum; PCho increases
  C. upsaliensis susceptibility, while MeOPN provides protection. These results
  suggest that phosphorylated surface modifications play key roles in C. upsaliensis
  host survival as well as pathogenesis.

• Subjects / Keywords

  • Serum suceptibility
  • Adherence and invasion
  • Phosphoramidate
  • Pathogenesis
  • Campylobacter upsaliensis
  • Phosphocholine

• Graduation date

  Fall 2012

• Type of Item

  Thesis

• Degree

  Master of Science

• DOI

  https://doi.org/10.7939/R3XK7R

• License

  This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.