Gene-expression profiling of calves 6 and 9 months after inoculation with Mycobacterium avium subspecies paratuberculosis

• Author(s) / Creator(s)

  • David, Joel
  • De Buck, Jeroen
  • Barkema, Herman W.
  • Guan, Leluo

• Early detection of Johne's disease (JD) caused by Mycobacterium avium subspecies paratuberculosis (MAP) is essential to reduce transmission; consequently, new diagnostic techniques and approaches to detect MAP or markers of early MAP infection are being explored. The objective was to identify biomarkers associated with MAP infection at 6 and 9 months after oral inoculation. Therefore, gene expression analysis was done using whole blood cells obtained from MAP-infected calves. All MAP-inoculated calves had a cell-mediated immune response (IFN-γ) to Johnin PPD specific antigens, and 60% had an antibody response to MAP antigens. Gene expression analysis at 6 months after inoculation revealed downregulation of chemoattractants, namely neutrophil beta-defensin-9 like peptide (BNBD9-Like), S100 calcium binding protein A9 (s100A9) and G protein coupled receptor 77 (GPR77) or C5a anaphylatoxin chemotactic receptor (C5a2). Furthermore, BOLA/MHC-1 intracellular antigen presentation gene was downregulated 9 months after inoculation. In parallel, qPCR experiments to evaluate the robustness of some differentially expressed genes revealed consistent downregulation of BOLA/MHC-I, BNBD9-Like and upregulation of CD46 at 3, 6, 9, 12, and 15 months after inoculation. In conclusion, measuring the expression of these genes has potential for implementation in a diagnostic tool for the early detection of MAP infection.

• Date created

  2014

• Subjects / Keywords

  • Lipid Mediators
  • Dendritic Cells
  • T-Lymphocyte Migration
  • Johnes-Disease
  • Real-Time Pcr
  • SSP Paratuberculosis
  • Blood Mononuclear-Cells
  • Crohns-Disease
  • Antigen Presentation
  • Growth-Factor-Beta

• Type of Item

  Article (Published)

• DOI

  https://doi.org/10.7939/R3VH5CX67

• License

  Attribution 4.0 International