The potential of novel small inhibitory molecules to prevent the rejection of neonatal porcine islets in mice

  • Author / Creator
    Mihalicz, Dana
  • The objectives of this study were to determine the effect of small inhibitory molecules suramin and DR80 on T cell proliferation in vitro as well as in vivo by preventing the rejection of neonatal porcine islets (NPI) in mice when combined with anti-LFA-1 monoclonal antibody (mAb). Suramin and DR80 inhibited T cell proliferation in vitro in a dose dependent manner with minimal cytotoxicity. As a monotherapy, neither suramin, DR80, nor anti-LFA-1 mAb prevented the rejection of NPI transplants. One of seven mouse recipients treated with suramin and anti-LFA-1 mAb and 6/10 mouse recipients treated with DR80 and anti-LFA-1 mAb achieved normoglycemia for more than 100 days after transplantation; 1 of the mice in the latter group subsequently rejected the NPI transplant. In conclusion, our findings indicates that the combination of DR80 and anti-LFA-1 mAb may be a promising therapy to prevent rejection of NPI in individuals with type 1 diabetes.

  • Subjects / Keywords
  • Graduation date
    Fall 2011
  • Type of Item
  • Degree
    Master of Science
  • DOI
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
  • Institution
    University of Alberta
  • Degree level
  • Department
  • Supervisor / co-supervisor and their department(s)
  • Examining committee members and their departments
    • Rajotte, Ray (Surgery)
    • Williams, David (Surgery)
    • Baldwin, Troy (Medical Microbiology and Immunology)