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Affinities of recombinant norovirus P dimers for human blood group antigens

  • Author(s) / Creator(s)
  • Noroviruses (NoVs), the major cause of viral acute gastroenteritis, recognize histo-blood group antigens (HBGAs) as receptors or attachment factors. To gain a deeper understanding of the interplay between NoVs and their hosts, the affinities of recombinant P dimers (P2's) of a GII.4 NoV (VA387) to a library of 41 soluble analogs of HBGAs were measured using the direct electrospray ionization mass spectrometry assay. The HBGAs contained the A, B, H and Lewis epitopes, with variable sizes (2–6 residues) and different types (1–6). The results reveal that the P2's exhibit a broad specificity for the HBGAs and bind to all of the oligosaccharides tested. Overall, the affinities are relatively low, ranging from 400 to 3000 M−1 and are influenced by the chain type: 3 > 1 ≈ 2 ≈ 4 ≈ 5 ≈ 6 for H antigens; 6 > 1 ≈ 3 ≈ 4 ≈ 5 > 2 for A antigens; 3 > 1 ≈ 4 ≈ 5 ≈ 6 > 2 for B antigens, but not by chain length. The highest-affinity ligands are B type 3 (3000 ± 300 M−1) and A type 6 (2350 ± 60 M−1). While the higher affinity to the type 3 H antigen was previously observed, preferential binding to the types 6 and 3 antigens with A and B epitopes, respectively, has not been previously reported. A truncated P domain dimer (lacking the C-terminal arginine cluster) exhibits similar binding. The central-binding motifs in the HBGAs were identified by molecular-docking simulations.

  • Date created
    2013
  • Subjects / Keywords
  • Type of Item
    Article (Published)
  • DOI
    https://doi.org/10.7939/R33J39D4G
  • License
    © 2015 Han, L., Kitov, P. I., Kitova, E. N., Tan, M., Wang, L., Xia, M., Jiang, X., & Klassen, J. S. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.
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  • Citation for previous publication
    • Han, L., Kitov, P. I., Kitova, E. N., Tan, M., Wang, L., Xia, M., Jiang, X., & Klassen, J. S. (2013). Affinities of recombinant norovirus P dimers for human blood group antigens. Glycobiology, 23(3), 276-285. http://doi.org/10.1093/glycob/cws141
  • Link to related item
    http://doi.org/10.1093/glycob/cws141