Affinities of recombinant norovirus P dimers for human blood group antigens

• Author(s) / Creator(s)

  • Klassen, John S.
  • Xia, Ming
  • Tan, Ming
  • Wang, Leyi
  • Han, Ling
  • Kitova, Elena N.
  • Jiang, Xi
  • Kitov, Pavel I.

• Noroviruses (NoVs), the major cause of viral acute gastroenteritis, recognize histo-blood group antigens (HBGAs) as receptors or attachment factors. To gain a deeper understanding of the interplay between NoVs and their hosts, the affinities of recombinant P dimers (P2's) of a GII.4 NoV (VA387) to a library of 41 soluble analogs of HBGAs were measured using the direct electrospray ionization mass spectrometry assay. The HBGAs contained the A, B, H and Lewis epitopes, with variable sizes (2–6 residues) and different types (1–6). The results reveal that the P2's exhibit a broad specificity for the HBGAs and bind to all of the oligosaccharides tested. Overall, the affinities are relatively low, ranging from 400 to 3000 M−1 and are influenced by the chain type: 3 > 1 ≈ 2 ≈ 4 ≈ 5 ≈ 6 for H antigens; 6 > 1 ≈ 3 ≈ 4 ≈ 5 > 2 for A antigens; 3 > 1 ≈ 4 ≈ 5 ≈ 6 > 2 for B antigens, but not by chain length. The highest-affinity ligands are B type 3 (3000 ± 300 M−1) and A type 6 (2350 ± 60 M−1). While the higher affinity to the type 3 H antigen was previously observed, preferential binding to the types 6 and 3 antigens with A and B epitopes, respectively, has not been previously reported. A truncated P domain dimer (lacking the C-terminal arginine cluster) exhibits similar binding. The central-binding motifs in the HBGAs were identified by molecular-docking simulations.

• Date created

  2013

• Subjects / Keywords

  • Affinity
  • Electrospray ionization mass spectrometry
  • Receptor
  • Histo-blood group antigens
  • Norovirus

• Type of Item

  Article (Published)

• DOI

  https://doi.org/10.7939/R33J39D4G

• License

  © 2015 Han, L., Kitov, P. I., Kitova, E. N., Tan, M., Wang, L., Xia, M., Jiang, X., & Klassen, J. S. This version of this article is open access and can be downloaded and shared. The original author(s) and source must be cited.