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Inheritance of peroxisomes in the yeast Yarrowia lipolytica

  • Author / Creator
    Chang, Jinlan
  • Peroxisomes are indispensable organelles that perform many essential metabolic activities. Thus, eukaryotic cells have evolved molecular mechanisms to ensure the inheritance of peroxisomes from mother cell to daughter cell at cell division. In the budding yeast Saccharomyces cerevisiae, the class V myosin motor, Myo2p, interacts with its peroxisomal receptor, Inp2p, to move peroxisomes along actin from mother cell to bud, while the peroxisomal membrane protein Inp1p functions to tether peroxisomes to the cell cortex. In this thesis, I report the results of investigations of peroxisome inheritance using the dimorphic yeast Yarrowia lipolytica as a model system. We showed that peroxisome mobility and inheritance are dependent on actin in Y. lipolytica. Interrogation of the Y. lipolytica genome revealed one class V myosin. This myosin V is involved in transporting peroxisomes from mother cell to bud. We characterized YlInp1p, the othologue of S. cerevisiae Inp1p, as the first peroxisomal protein required for peroxisome inheritance in Y. lipolytica. We demonstrated that YlInp1p functions to anchor peroxisomes in both mother cell and bud. YlInp1p has an additional role in the dimorphic transition from the yeast form to the hyphal form in Y. lipolytica. We identified Pex3Bp, a paralogue of Pex3p, as the peroxisome-specific receptor for myosin V in Y. lipolytica. Pex3Bp interacts directly with the globular tail of myosin V. Pex3Bp also interacts with itself and with Pex3p. In cells lacking Pex3Bp, peroxisomes are preferentially retained in the mother cell, while the majority of peroxisomes gather and are transferred to the bud in cells overproducing Pex3Bp. Overexpression of PEX3 can partially complement the phenotype of pex3B∆ cells, while overexpression of PEX3B cannot complement the phenotype of pex3∆ cells. Interestingly, Pex3p, which has been shown previously to function in the de novo formation of peroxisomes from the ER, also interacts directly with the globular tail of myosin V. Therefore, Pex3p is involved in peroxisome inheritance. In addition, cells lacking Pex3Bp contain hyperelongated, tubulo-reticular peroxisomes, indicating that Pex3Bp has a role in peroxisome morphology. Our findings suggest that both Pex3Bp and Pex3p are multifunctional proteins that are involved in different steps of the peroxisome biogenic cascade.

  • Subjects / Keywords
  • Graduation date
    2010-11
  • Type of Item
    Thesis
  • Degree
    Doctor of Philosophy
  • DOI
    https://doi.org/10.7939/R3MT0Q
  • License
    This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for non-commercial purposes. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
  • Language
    English
  • Institution
    University of Alberta
  • Degree level
    Doctoral
  • Department
    • Department of Cell Biology
  • Supervisor / co-supervisor and their department(s)
    • Rachubinski, Richard (Cell Biology)
  • Examining committee members and their departments
    • Mullen, Robert (University of Guelph)
    • Casey, Joseph (Physiology)
    • Wang, Zhixiang (Cell Biology)
    • Eitzen, Gary (Cell Biology)