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Studying the effects of mouse prion protein on mice and rat colon antibacterial gene expression profiling Open Access


Other title
antibacterial gene expression profiling
prion protein
Type of item
Degree grantor
University of Alberta
Author or creator
Lam, Tran Hu Th
Supervisor and department
Ametaj, Burim N. (Agricultural, Food, and Nutritional Science)
Examining committee member and department
Gänzle, Michael (Agricultural, Food, and Nutritional Science)
Guan, Leluo (Agricultural, Food, and Nutritional Science)
Department of Agricultural, Food, and Nutritional Science
Animal Science
Date accepted
Graduation date
Master of Science
Degree level
The objectives of this thesis research are: 1) to evaluate whether there is interspecies translocation of mouse recombinant prion protein (moPrP) through colon tissue of rats; 2) to investigate whether lipopoplysaccharide (LPS) from Escherichia coli influences permeability of moPrP through the colon tissue of mice; 3) to assess whether mouse (mo)PrP affects antibacterial gene expression profile in the colon of rats and mice; 4) to test whether combinations of moPrP with LPS or detoxified LPS (D-LPS) are able to affect genes related to antibacterial response; 5) to examine whether presence of LPS on the mucosal or serosal side of the colon tissues will affect permeability of moPrP and gene expression profiles in the colon. Overall, moPrP alone or in combination with LPS or D-LPS did not translocate through the intact colon tissues of rats or mice. moPrP alone or combined with LPS or D-LPS on the mucosal or serosal sides of diffusion chamber affected expression of various genes related to inflammation and antibacterial response.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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