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Permanent link (DOI): https://doi.org/10.7939/R33N20N70

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All-Optical and Endoscopic Photoacoustic Microscopy Open Access

Descriptions

Other title
Subject/Keyword
Remote sensing
PARS
Photoacoustic
Optical imaging
Imaging
in vivo
Optical Resolution
Biomedical, Optics
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Haji Reza, Parsin
Supervisor and department
Roger Zemp
Examining committee member and department
Mike Brett (ECE)
Michael C. Kolios (External examiner, physics, ryerson)
Ying Tsui (ECE)
Mojgan Daneshmand (chair, ECE)
Vien Van(ECE)
Robert Fedosejevs (ECE)
Department
Department of Electrical and Computer Engineering
Specialization
Biomedical Engineering
Date accepted
2015-08-25T10:35:40Z
Graduation date
2015-11
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
Optical-resolution photoacoustic microscopy (OR-PAM) is a novel imaging technology providing high micron-scale lateral spatial resolution to visualize superficial structures in vivo with optical-absorption contrast. The term “optical resolution” is used since the lateral resolution of the system is defined by the optically-focused spot size, which is limited by the diffraction-limit of light. The axial resolution of the system is still inversely related to the bandwidth of transducer, typical of other photoacoustic microscopy (PAM) systems. Due to limitations of light transport, OR-PAM is used for imaging superficial structures to depths of about 1 mm in tissue. OR-PAM is able to image capillary networks and quantify morphological and functional parameters such as number of vessels, diameter and length of the vessels, total hemoglobin concentration and hemoglobin oxygen saturation. A tumor needs to develop angiogenesis in order to consume more oxygen and nutrients to grow. These blood vessels can provide imaging contrast for photoacoustic imaging, making OR-PAM a useful tool for tumor imaging. However there are several limitations with current OR-PAM systems. One of the limitations is their lack of flexibility. The present OR-PAM systems are mostly mounted as a table-top device with a large footprint. In this dissertation we introduce a label free optical-resolution photoacoustic micro-endoscopy to enable access to internal body cavities and a flexible handheld probe. Also, a multi-wavelength fiber laser with tunable repetition rates as high as 600 kHz, and high pulse-to pulse stability is demonstrated for in vivo real time functional imaging. This has been done by taking advantage of stimulated Raman scattering (SRS) in a single mode fiber. This system is also modified to perform a multi-focus OR-PAM by taking advantage of chromatic aberration in the collimating/objective lens pair to refocus light from a fiber into an object so that each iii wavelength is focused at a slightly different depth location. Design and fabrication of a novel Fabry-Perot etalon- based ultrasound detectors by using glancing angle deposition (GLAD) nanostructured thin-films is shown as well. Finally a novel all optical non-contact photoacoustic remote sensing (PARS) microscopy system is demonstrated with a lateral resolution as low as 2 μm. This system offers optical absorption contrast without the need of bulky ultrasound transducers or coupling medium and can provide performance comparable to previous OR-PAM systems but in a non-contact setting.
Language
English
DOI
doi:10.7939/R33N20N70
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
P. Hajireza, W. Shi, and R. J. Zemp, "Label-free in vivo fiber-based optical-resolution photoacoustic microscopy," Opt. Lett. 36, 4107-4109 (2011) (also Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics).P. Hajireza, W. Shi and R. J. Zemp, "Label-Free In vivo GRIN-Lens Optical Resolution Photoacoustic Micro-Endoscopy ", Laser Phys. Lett. 10 055603 (2013)Parsin Hajireza, Tyler Harrison, Alexander Forbrich and Roger Zemp "Optical resolution photoacoustic microendoscopy with ultrasound-guided insertion and array system detection", J. Biomed. Opt. 18(9), 090502 (2013).Parsin Hajireza, Wei Shi, and Roger J. Zemp, "Real-time handheld optical-resolution photoacoustic microscopy," Opt. Express 19, 20097-20102 (2011) ( also Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics ).Parsin Hajireza, Alexander Forbrich and Roger J. Zemp" In-vivo functional optical-resolution photoacoustic microscopy with stimulated Raman scattering fiber-laser source" Biomed. Opt. Express 5, 539-546 (2014).Parsin Hajireza, Alexander Forbrich, and Roger J. Zemp, "Multifocus optical-resolution photoacoustic microscopy using stimulated Raman scattering and chromatic aberration," Opt. Lett. 38, 2711-2713 (2013).Parsin Hajireza, Kathleen Krause, Michael Brett, and Roger Zemp, "Glancing angle deposited nanostructured film Fabry-Perot etalons for optical detection of ultrasound," Optics Express 21, 6391-6400 (2013) (also Vol. 8, Iss. 4 Virtual Journal for Biomedical Optics).Parsin Hajireza, Jason Sorge, Michael Brett, and Roger Zemp, "In vivo optical resolution photoacoustic microscopy using glancing angle-deposited nanostructured Fabry–Perot etalons," Opt. Lett. 40, 1350-1353 (2015)

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