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Examination of Immunoregulatory Receptors in the Channel Catfish (Ictalurus punctatus) Open Access


Other title
Immunoregulatory receptors
Cellular signaling
Comparative immunology
Innate immunity
Channel catfish
Type of item
Degree grantor
University of Alberta
Author or creator
Cortes, Herman D
Supervisor and department
Stafford, James (Biological Sciences)
Examining committee member and department
Magor, Kathy (Biological Sciences)
Tierney, Keith (Biological Sciences)
Hazes, Bart (Medical Microbiology & Immunology)
Department of Biological Sciences
Physiology, Cell and Developmental Biology
Date accepted
Graduation date
Master of Science
Degree level
In response to pathogens, immune cells induce protective effector functions, such as degranulation, phagocytosis and cytokine secretion, which are initiated by stimulatory or inhibitory immunoregulatory receptors expressed on leukocytes. Using a fish immunological model system, the focus of my thesis work was to examine immunoregulatory receptors in vertebrates by further characterizing members of the teleost Leukocyte Immune-type Receptor (LITR) family. IpLITRs share structural and phylogenetic features with certain mammalian receptors involved in activating and abrogating immune cell functions. As stimulatory IpLITR2.6b associates with signaling adaptor molecule IpFcRγ-L, I expressed a chimeric construct of IpLITR2.6b with IpFcRγ-L in mammalian immune cells to examine its functional abilities. Engagement of this receptor- adaptor induced intracellular signaling and activation of degranulation and phagocytosis. I also expressed IpLITR1.1b, a receptor previously reported to inhibit natural killer cell cytotoxicity to examine its functions further. This inhibitory receptor surprisingly facilitated phagocytosis. This represents dual functionality among select IpLITR-types, which, to our knowledge, has not been reported before in fish. Taken together, these results highlight conserved aspects of immunoregulatory receptor signaling and function while revealing new insights that may contribute to our overall understanding of immunity.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Cortes, et al. 2012. Dev Comp Immunol. 36: 62-­‐73.Montgomery, et al. 2011. Dev Comp Immunol. 35: 1223-­‐37.

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