Download the full-sized PDF of A Serological Diagnosis of Celiac Disease: A Pilot Study Toward Changing Local PracticeDownload the full-sized PDF



Permanent link (DOI):


Export to: EndNote  |  Zotero  |  Mendeley


This file is in the following communities:

Graduate Studies and Research, Faculty of


This file is in the following collections:

Theses and Dissertations

A Serological Diagnosis of Celiac Disease: A Pilot Study Toward Changing Local Practice Open Access


Other title
Celiac Disease
serological diagnosis
Type of item
Degree grantor
University of Alberta
Author or creator
Rajani, Seema
Supervisor and department
Turner, Justine (Paediatrics)
Persad, Rabin (Paediatrics)
Examining committee member and department
Bamforth, Fiona (Medical Genetics)
Huynh, Hien (Paediatrics)
Rempel, Gwen (Nursing)
Persad, Sujata (Paediatrics)
Medical Sciences-Paediatrics

Date accepted
Graduation date
Master of Science
Degree level
Introduction: Celiac disease (CD) is the most common autoimmune disorder of the gastrointestinal tract. In 2012, the European Society for Pediatric Gastroenterology, Hepatology and Nutrition changed their guidelines for CD diagnosis to include a serological diagnosis for certain patients instead of an endoscopy followed by biopsy. The purpose of this study is: (1) to apply a modified serological protocol in a prospective manner, (2) to evaluate the use of non-invasive monitoring of mucosal damage and (3) conduct qualitative interviews to determine if diagnostic strategy affects outcome, on a gluten free diet (GFD). Methods: Pediatric patients were given the option of a serological diagnosis if their anti-tissue transglutaminase (aTTG) level was ≥200 U/mL. Those that had an aTTG <200 U/mL were diagnosed by biopsy. In both groups, intestinal permeability and inflammation were assessed using standard non-invasive measurements of sugar probes in urine and fecal calprotectin. Parental phone interviews were also conducted in a subset of each diagnostic group. Conclusions: Our enrollment rate and parent response in interviews demonstrate that, in our local center, parents and patients welcome a non-invasive diagnostic strategy. There were no adverse affects in regards to symptom improvement or adherence to the GFD in those diagnosed by serology. After 12 months of treatment on a gluten-free diet, all CD patients showed recovery in intestinal permeability and inflammation through non-invasive measurements.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
File format: pdf (PDF/A)
Mime type: application/pdf
File size: 2278840
Last modified: 2015:10:21 00:54:59-06:00
Filename: Rajani_Seema_201501_MSc.pdf
Original checksum: 9e0789d26c9360ae128944cdace0e9de
Activity of users you follow
User Activity Date