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Permanent link (DOI): https://doi.org/10.7939/R3V97ZX40

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Detection and Characterization of Orthoreovirus in Alberta's Environmental Waters Open Access

Descriptions

Other title
Subject/Keyword
Mammalian orthoreovirus
Reovirus
Orthoreovirus
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Kostiuk, Tyler T.
Supervisor and department
Pang, Xiao-Li (Laboratory Medicine and Pathology)
Neumann, Norman (Public Health)
Examining committee member and department
Neumann, Normann (Public Health)
Pang, Xiao-Li (Laboratory Medicine and Pathology)
Preiksaitis, Jutta (Medicine)
Lee, Bonita (Pediatrics)
Shmulevitz, Maya (Medical Microbiology and Immunology)
Department
Medical Sciences-Laboratory Medicine and Pathology
Specialization

Date accepted
2015-09-22T15:51:51Z
Graduation date
2015-11
Degree
Master of Science
Degree level
Master's
Abstract
Reoviruses have been shown to infect a very broad range of mammalian sources and infections in humans have been associated with gastrointestinal and respiratory illnesses. While the presence of reovirus in several different water matrices and clinical samples has been reported, the clinical implications of reovirus in Alberta’s water and clinical samples have not been studied because of the lack of an appropriate screening method. To improve reovirus detection, a novel reverse-transcription real time PCR (qRT-PCR) assay was designed, developed, and implemented. Two sets of novel qPCR primers and probes (targeting the M1 and L3 genetic fragments) were designed and found to have sensitivities of 5 genetic copies per qPCR for M1 and 50 genetic copies per qPCR for L1, respectively. Using the newly developed qRT-PCR assay, reoviruses were detected in wastewater (15/16) and environmental surface water (20/216) samples obtained from various sites in Alberta, while no reoviruses were observed in clinical fecal samples obtained from patients suffering from gastroenteritis. Phylogenetic trees produced from S4 gene fragment sequences indicate that a variety of S4 alleles are present in the environment in Alberta, which is consistent with data previously published regarding reoviruses in the environment.
Language
English
DOI
doi:10.7939/R3V97ZX40
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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