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Permanent link (DOI): https://doi.org/10.7939/R3QN5ZP87

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Genotoxicity and Mutagenicity of Extractable Organics from Oil Sands Process-Affected Water Open Access

Descriptions

Other title
Subject/Keyword
OSPW
SOS-chromo test
Ames
Mutagenicity,
Genotoxicity
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Zetouni, Nikolas Cavalheiro
Supervisor and department
Jonathan W Martin, Medical Sciences - Laboratory Medicine and Pathology
Examining committee member and department
Arno Siraki, Department of Pharmacy and Pharmaceutical Sciences
Michael Weinfeld, Department of Oncology
Department
Medical Sciences-Laboratory Medicine and Pathology
Specialization

Date accepted
2015-07-23T15:49:25Z
Graduation date
2015-11
Degree
Master of Science
Degree level
Master's
Abstract
Oil sands process-affected water (OSPW) is produced in the surface mining industry during the hot-water extraction of bitumen from oil-sands ore. Its acid extractable organic (AEO) fraction is known to be acutely toxic, but few studies have addressed its genotoxicity or mutagenicity. Here, the in vitro SOS-Chromo genotoxicity test and Ames test (TA98 and TA100) were used to evaluate these endpoints for the whole AEO mixture and two chemical sub-fractions (acid and neutral extractable), with and without S9 enzymes. Whole AEO showed increased genotoxicity at concentrations above 7x (SOS response of 9.63±2.11SD) where S9 fraction decreased genotoxicity by 70%. AEO mutagenicity in the TA 98 strain was similar to the TA100 strain and S9 generally decreased the mutagenicity. Genotoxicity of the acid extractable subfraction was lower than whole AEOs, with or without S9, while the neutral extractable subfraction SOS-response was the lowest and S9 presence did not significantly decrease genotoxicity. The acid extractable subfraction mutageniticy was also similar in TA98 and TA100, and S9 decreased mutagenicity by approximately half, with the TA 100 strain showing the highest mutagenicity. OSPW AEOs are genotoxic and mutagenic in the in vitro assays used here, but the mechanisms are in need of clarification and further studies examining the carcinogenic potential of OSPW are warranted.
Language
English
DOI
doi:10.7939/R3QN5ZP87
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Zetouni NC, Siraki A, Weinfeld M, Pereira, A and Martin, JW. (2015). Screening of Genotoxicity and Mutagenicity in Extractable Organics from Oil Sands Process-Affected Water. Environmental Toxicology and Chemistry. In press

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