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Signal processing and amplifier design for inexpensive genetic analysis instruments Open Access


Other title
wavelet transform
multistage amplifier
capillary electrophoresis
Jansson's deconvolution
overlapping peak separation
signal processing
peak detection
Type of item
Degree grantor
University of Alberta
Author or creator
Choi, Sheng Heng
Supervisor and department
Gaudet, Vincent (Electrical and Computer Engineering)
Backhouse, Chris (Electrical and Computer Engineering)
Examining committee member and department
Bowman, John (Mathematics and Statistics)
Cockburn, Bruce (Electrical and Computer Engineering)
Electrical and Computer Engineering

Date accepted
Graduation date
Master of Science
Degree level
The Applied Miniaturisation Laboratory (AML) has recently built a laser-induced fluorescent capillary electrophoresis (LIF-CE) genetic analysis instrument, called the Tricorder Tool Kit (TTK). By using a photodiode instead of photomultiplier tubes in the optical detection, the AML has lowered the cost and size compared to commercial LIF-CE products. However, maintaining an adequate signal-to-noise (SNR) and limit of detection (LOD) is a challenge. By implementing a multistage amplifier, we increased the bandwidth and voltage swing while maintaining the transimpedance gain compared to the previous design. We also developed signal processing algorithms for post-experiment processing of CE. Using wavelet transform, iterative polynomial baseline fitting, and Jansson's deconvolution, we improved the SNR, reduced baseline variations, and separated overlapping peaks in CE signals. By improving the electronics and signal processing, we lowered the LOD of the TTK, which is a step towards the realisation of inexpensive point-of-care molecular medical diagnosis instruments.
License granted by Sheng Heng Choi ( on 2011-07-07 (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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