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Permanent link (DOI): https://doi.org/10.7939/R3QK88

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Changes in ruminal bacterial communities of beef cattle during high concentrated diet transition and experimental induced subacute acidosis Open Access

Descriptions

Other title
Subject/Keyword
rapid dietary transitions
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Yanhong, Chen
Supervisor and department
Dr. Leluo Guan (Agricultural, Food and Nutritional Science)
Examining committee member and department
Dr. Leluo Guan (Agricultural, Food and Nutritional Science)
Department
Department of Agricultural, Food, and Nutritional Science
Specialization

Date accepted
2011-08-31T17:14:25Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
Rapid dietary transition is a common practice in the nutritional management of feedlot cattle, which may lead to ruminal acidosis in some animals. Ruminal acidosis is a prevenient digestive disorder in livestock industry which significantly impacts animal health and production. To date, the understanding of the changes in the ruminal microbial community during diet transition or acidosis is very limited. In this study, we have investigated: 1) the epithelial tissue associated bacterial diversity changes in the rumen of beef cattle during dietary transition to high grain diets; and 2) variation of digesta and epithelium attached bacterial communities in the rumen of steers differing in susceptibility to subacute ruminal acidosis using culture-independent molecular based methods. PCR- denaturing gradient gel electrophoresis and quantitative real time PCR analysis of 24 beef heifers adapted from a diet containing 97% hay to the diet containing 8% hay over 29 days revealed that the epimural bacterial diversity from rapid grain adaptation heifers changed in response to the dietary transition. Similar analysis of bacterial profiles of rumen samples from acidosis-resistant and acidosis-susceptible steers showed that the diversity and density of digesta and epithelial attached bacterial communities are different between AS and AR animals.
Language
English
DOI
doi:10.7939/R3QK88
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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