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Changes in ruminal bacterial communities of beef cattle during high concentrated diet transition and experimental induced subacute acidosis Open Access


Other title
rapid dietary transitions
Type of item
Degree grantor
University of Alberta
Author or creator
Yanhong, Chen
Supervisor and department
Dr. Leluo Guan (Agricultural, Food and Nutritional Science)
Examining committee member and department
Dr. Leluo Guan (Agricultural, Food and Nutritional Science)
Department of Agricultural, Food, and Nutritional Science

Date accepted
Graduation date
Master of Science
Degree level
Rapid dietary transition is a common practice in the nutritional management of feedlot cattle, which may lead to ruminal acidosis in some animals. Ruminal acidosis is a prevenient digestive disorder in livestock industry which significantly impacts animal health and production. To date, the understanding of the changes in the ruminal microbial community during diet transition or acidosis is very limited. In this study, we have investigated: 1) the epithelial tissue associated bacterial diversity changes in the rumen of beef cattle during dietary transition to high grain diets; and 2) variation of digesta and epithelium attached bacterial communities in the rumen of steers differing in susceptibility to subacute ruminal acidosis using culture-independent molecular based methods. PCR- denaturing gradient gel electrophoresis and quantitative real time PCR analysis of 24 beef heifers adapted from a diet containing 97% hay to the diet containing 8% hay over 29 days revealed that the epimural bacterial diversity from rapid grain adaptation heifers changed in response to the dietary transition. Similar analysis of bacterial profiles of rumen samples from acidosis-resistant and acidosis-susceptible steers showed that the diversity and density of digesta and epithelial attached bacterial communities are different between AS and AR animals.
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