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Identification of dehydration tolerance genes in triticale (x Triticosecale Wittm.) seedlings Open Access


Other title
plant science
dehydration stress
Type of item
Degree grantor
University of Alberta
Author or creator
Badea, Cosmin
Supervisor and department
Weselake, Randall (AFNS), Laroche, André (AAFC)
Examining committee member and department
Michael Deyholos (Biological Science)
Laroche, André (AAFC)
King, Jane (AFNS) Chair
Weselake, Randall (AFNS)
Kav, Nataraj (AFNS)
Department of Agricultural, Food, and Nutritional Science
Plant Science
Date accepted
Graduation date
Master of Science
Degree level
Dehydration stress triggers many changes in plant gene expression. Triticale (x Triticosecale Wittm.) sequencing using next generation sequencing allowed identification of 107,000 transcripts of which 4,467 were differentially regulated ≥2-fold. Abscisic acid co-receptors, pyrabactin and protein phosphatases 2C were highly regulated and identified for the first time in dehydration stress. Many ABA-independent genes such as DREB/CBFs family were down-regulated or non-differentially expressed compared to non-stressed plants. This suggested that the solid polyethylene glycol system successfully led to identification of dehydration stress-specific genes from other stresses. Only 5% from the 87% annotated differentially regulated transcripts showed homology to previously identified dehydration responsive genes in wheat, maize, barley or Arabidopsis. Thus, this study revealed a larger number of novel genes potentially involved in dehydration stress, many of which were highly differentially regulated. Identification of differentially expressed genes and their functions during dehydration stress is essential towards improvement of dehydration tolerance crops.
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File title: Badea_Cosmin_Spring_2012.pdf
File title: Identification of drought tolerant genes in triticale (x Triticosecale Wittm.) seedlings
File author: Laroche
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