Download the full-sized PDF
Permanent link (DOI): https://doi.org/10.7939/R3M32NQ10
This file is in the following communities:
|Agricultural, Food and Nutritional Science, Department of|
This file is in the following collections:
|Journal Articles (Agricultural, Food and Nutritional Science)|
Potential regulatory role of microRNAs in the development of bovine gastrointestinal tract during early life Open Access
- Author or creator
McFadden, Thomas B.
Griebel, Philip J.
- Additional contributors
- Type of item
- Journal Article (Published)
This study aimed to investigate the potential regulatory role of miRNAs in the development of gastrointestinal tract (GIT) during the early life of dairy calves. Rumen and small intestinal (mid-jejunum and ileum) tissue samples were collected from newborn (30 min after birth; n = 3), 7-day-old (n = 6), 21-day-old (n = 6), and 42-day-old (n = 6) dairy calves. The miRNA profiling was performed using Illumina RNA-sequencing and the temporal and regional differentially expressed miRNAs were further validated using qRT-PCR. Analysis of 16S rRNA gene copy numbers was used to quantify total bacteria, Bifidobacterium and Lactobacillus species. The expression of miR-143 was abundant in all three gut regions, at all time points and it targets genes involved primarily in the proliferation of connective tissue cells and muscle cells, suggesting a role in regulating rapid tissue development during the early life of calves. The expression of miR-146, miR-191, miR-33, miR-7, miR-99/100, miR-486, miR-145, miR-196 and miR-211 displayed significant temporal differences (FDR <0.05), while miR-192/215, miR-194, miR-196, miR-205 and miR-31 revealed significant regional differences (FDR <0.05). The expression levels of miR-15/16, miR-29 and miR-196 were positively correlated with the copy numbers of 16S rRNA gene of Bifidobacterium or Lactobacillus species or both (P<0.05). Functional analysis using Ingenuity Pathway Analysis identified the above mentioned differentially expressed miRNAs as potential regulators of gut tissue cell proliferation and differentiation. The bacterial density-associated miRNAs were identified as modulators of the development of lymphoid tissues (miR-196), maturation of dendritic cells (miR-29) and development of immune cells (miR-15/16). The present study revealed temporal and regional changes in miRNA expression and a correlation between miRNA expression and microbial population in the GIT during the early life, which provides further evidence for another mechanism by which host-microbial interactions play a role in regulating gut development.
- Date created
- License information
- Attribution 4.0 International
- Citation for previous publication
Liang, G., Malmuthuge, N., McFadden, T. B., Bao, H., Griebel, P. J., Stothard, P., & Guan, L. L. (2014). Potential regulatory role of microRNAs in the development of bovine gastrointestinal tract during early life. PLoS ONE, 9(3), e92592 [13 pages]. http://dx.doi.org/10.1371/journal.pone.0092592
- Link to related item
- Date Uploaded
- Date Modified
- Audit Status
- Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 1062410
Last modified: 2017:09:06 16:42:26-06:00
Original checksum: 6f6f6590b70a05425119ea8eefb28f94
Well formed: true
File title: pone.0092592 1..13
Page count: 13