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Permanent link (DOI): https://doi.org/10.7939/R38K7524R

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Importance of donor factors and whole blood processing in the storage injury of red blood cells Open Access

Descriptions

Other title
Subject/Keyword
Transfusion medicine
Donor Factors
Blood manufacturing
Storage injury
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Jordan, Andrew M
Supervisor and department
Acker, Jason (Laboratory Medicine and Pathology)
Examining committee member and department
Holovati, Jelena (Laboratory Medicine and Pathology)
Nahirniak, Susan (Laboratory Medicine and Pathology)
Ward, Chris (Laboratory Medicine and Pathology)
Department
Medical Sciences-Laboratory Medicine and Pathology
Specialization

Date accepted
2016-09-27T09:55:36Z
Graduation date
2016-06:Fall 2016
Degree
Master of Science
Degree level
Master's
Abstract
Background: The processing and storage of blood components prior to transfusion are key parts of the blood banking industry. Recently, however, it has been suggested that the long term storage of red blood cells for transfusion could have detrimental clinical implications. In this study we investigate the pre-storage factors, such as donor characteristics and component processing conditions, that influence the way red blood cells change and accumulate injury during storage. Materials and Methods: Retrospective analysis of large scale blood bank databases was performed to identify sources of variability within stored red cell concentrates. Prospective storage studies were designed to further assess the effects of different pre-storage variables based on the observations of the retrospective data analysis. The volume of plasma and additive solution was measured in red cell concentrates processed using different methods; red blood cells were then stored in known volumes of plasma and additive solution to assess the impact on red cell storage injury. To investigate red blood cell turnover and senescence, red blood cell size and density was measured in stored red cell concentrates from different donor groups. Results: Component processing conditions and donor factors were both found to influence the post-storage characteristics of stored red cell concentrates. Buffy-coat component processed units exhibited less storage injury than units processed using the whole blood filtration method. The influence of component processing was found to be independent of the residual plasma volume and additive solution volume. Units from female donors exhibited less storage haemolysis than units from male donors, and increasing donor age was also associated with increasing storage haemolysis. Donor sex and age were shown to influence; pre-donation haemoglobin concentration, haematocrit, mean cell volume, cell density, cell haemoglobin content. Young female donors exhibited the lowest pre-donation haemoglobin concentrations, and the largest, least dense red blood cells. Conclusions: The effect of hypothermic storage on red blood cell characteristics is dependent not only on the duration of storage; but also the component manufacturing conditions the cells are exposed to, and the unique characteristics of the donor’s blood. Understanding how these variables generate definable populations of red cell products is a key step in providing the safest, most appropriate transfusion components to hospitals and patients.
Language
English
DOI
doi:10.7939/R38K7524R
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
Citation for previous publication
A. Jordan, D. Chen, Q. –L. Yi., T. Kanias, M. T. Gladwin, J. P. Acker, “Assessing the influence of component processing and donor characteristics on quality of red cell concentrates using quality control data.” Vox Sanguinis, 2016; 111(1):8-15.A. Jordan, J. P. Acker, “Determining the Volume of Additive Solution and Residual Plasma in Whole Blood Filtered and Buffy Coat Processed Red Cell Concentrates.” Transfusion Medicine and Hemotherapy, 2016; 43:133-136

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