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Permanent link (DOI): https://doi.org/10.7939/R3VM4387J

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Application of Low Intensity Pulsed Ultrasound for Microbial Cell Stimulation in Bioprocesses And Development of Carbon-based Silver Covered Filters for Microbial Cells Removal in Water Systems Open Access

Descriptions

Other title
Subject/Keyword
Renewable energy
Algae
Water filtration in greenhouses
LIPUS
Ultrasound
Bioprocesses
Microbial cells
Biotechnology
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Savchenko, Oleksandra
Supervisor and department
Chen, Jie (Electrical and Computer Engineering, Biomedical Engineering)
Examining committee member and department
Ma, Kesen (external, University of Waterloo)
Burrell, Robert (Biomedical Engineering)
Liu, Yang (Civil and Environmental Engineering)
Chang, Scott (Renewable Resources)
Chen, Jie (Electrical and Computer Engineering, Biomedical Engineering)
Li, Huazhou (Civil and Environmental Engineering)
Department
Department of Biomedical Engineering
Specialization

Date accepted
2017-03-30T13:58:27Z
Graduation date
2017-06:Spring 2017
Degree
Doctor of Philosophy
Degree level
Doctoral
Abstract
Renewable resources with their potential application in food industry, pharmacy, energy sector, chemistry, material science etc. can be a major player in our everyday lives. Apart from sustainability in the production, the use of bioprocesses in the energy production and waste water treatment can address the important problem of water, soil and air pollution as well as climate change, which can be essential for a higher level of life quality on the planet. Therefore, the potential use and modification of microorganisms, such as microalgae, in fermentation and as biocatalysts as well as new chemical, physical and biological methods to improve cost-efficiency of green and white biotechnology are gaining a lot of attention from modern research all over the world. One of the alternative approaches to apply in biotechnological production that can successfully improve cost-efficiency and increase yield can be ultrasound - a form of acoustic energy, characterized by frequencies of 20 kHz -1 GHz, or the range that is above the human hearing and lower than the hypersonic regime. Low-intensity pulsed ultrasound (1.5MHz, 20% duty cycle, and intensity between 20mW/cm2 and 200mw/cm2) has been previously used in the bioprocesses and has been proven to increase stem cell, microorganism and antibody growth. This research presents a study on the impact of Low Intensity Pulsed Ultrasound (LIPUS) as a physical method for microorganism growth and activity stimulation, which can be used in combination with all other biological and chemical methods in bioprocesses. The study showed it can be applied in algal biofuel production giving up to 20% increase in lipid yield, in bio-ethanol production using microorganisms with up to 20-30% improvement in sugars to ethanol conversion efficiency, for increasing biomass of microalgae up to 20% with potential use for food, material and pharmaceutical applications, as well as for waste-water treatment with microorganisms. Improving the efficiency of these processes by this mechanism on an industrial scale could yield more affordable price for biofuel or other products of above mentioned bioprocesses. The study has also revealed that LIPUS showed consistent results in different bioprocesses with different microorganisms and duration of cell culture, which indicates that there is a broad spectrum of potential LIPUS applications.
Language
English
DOI
doi:10.7939/R3VM4387J
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
Citation for previous publication
Oleksandra Savchenko, Jida Xing, Xiaoyan Yang, Quanrong Gu, Mohamed Shaheen, Min Huang, Xiaojian Yu, Robert Burrell, Prabir Patra and Jie Chen. “ Algal Cell Response to Pulsed Waved Stimulation and Its Application to Increase Algal Lipid Production” Scientific Reports 7, Article number: 42003 (2017)Oleksandra Savchenko, Jie Chen, Yuzhi Hao, Xiaoyan Yang, Susie Li and Jian Yang, “ Three-Dimensional Coating of Porous Activated Carbons with Silver Nanoparticles and its Scale-up Design for Plant Disease Management in Greenhouses“ J Plant Pathol Microbiol 2016, 7: 381. 7:10, (2016)

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