Download the full-sized PDF
Permanent link (DOI): https://doi.org/10.7939/R3HX1645W
This file is in the following communities:
|Agricultural, Food and Nutritional Science, Department of|
This file is in the following collections:
|Journal Articles (Agricultural, Food and Nutritional Science)|
Microarray analysis in caudal medulla of cattle orally challenged with bovine spongiform encephalopathy Open Access
- Author or creator
Almeida, Luciane M.
Williams, John L.
Moore, Stephen S.
- Additional contributors
Transmissible Spongiform Encephalopathy
- Type of item
- Journal Article (Published)
Bovine spongiform encephalopathy (BSE) is a fatal disorder in cattle characterized by progressive neurodegeneration of the central nervous system. We investigated the molecular mechanisms involved in neurodegeneration during prion infection through the identification of genes that are differentially expressed (DE) between experimentally infected and non-challenged cattle. Gene expression of caudal medulla from control and orally infected animals was compared by microarray analysis using 24,000 bovine oligonucleotides representing 16,846 different genes to identify DE genes associated with BSE disease. In total, 182 DE genes were identified between normal and BSE-infected tissues (>2.0-fold change, P < 0.01); 81 DE genes had gene ontology functions, which included synapse function, calcium ion regulation, immune and inflammatory response, apoptosis, and cytoskeleton organization; 13 of these genes were found to be involved in 26 different Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. The expression of five DE genes associated with synapse function (tachykinin, synuclein, neuropeptide Y, cocaine, amphetamine-responsive transcript, and synaptosomal-associated protein 25 kDa) and three DE genes associated with calcium ion regulation (parvalbumin, visinin-like, and cadherin) was further validated in the medulla tissue of cattle at different infection times (6, 12, 42, and 45 months post-infection) by qRT-PCR. These data will contribute to a better understanding of the molecular mechanisms of neuropathology in bovine species.
- Date created
- License information
- Attribution-NonCommerical-ShareALike 4.0 International
- Citation for previous publication
Almeida, L. M., Basu, U., Williams, J. L., Moore, S. S., & Guan, L. L. (2011). Microarray analysis in caudal medulla of cattle orally challenged with bovine spongiform encephalopathy. Genetics and Molecular Research, 10(4), 3948-3962. http://dx.doi.org/10.4238/2011.October.25.5
- Link to related item
- Date Uploaded
- Date Modified
- Audit Status
- Audits have not yet been run on this file.
File format: pdf (Portable Document Format)
Mime type: application/pdf
File size: 514855
Last modified: 2017:09:06 16:23:14-06:00
Original checksum: 1f4e28d7eea5f295596ec4790648c106
Well formed: true
Page count: 15