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Permanent link (DOI): https://doi.org/10.7939/R3J707

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Synthesis and SAR studies of antimicrobial peptide Leucocin A Open Access

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Other title
Subject/Keyword
antimicrobial peptide, leucocin a, bacteriocin
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Bodapati, Krishna Chaitanya
Supervisor and department
Dr.Kamaljit Kaur (pharmacy)
Examining committee member and department
Department
Faculty of Pharmacy and Pharmaceutical Sciences
Specialization

Date accepted
2011-09-01T21:45:20Z
Graduation date
2011-11
Degree
Master of Science
Degree level
Master's
Abstract
In this study, we report the synthesis of a potent antimicrobial peptide Leucocin A (LeuA) using two solid phase peptide synthesis methods. One of the methods, native chemical ligation, gave high yield (12.5%) of 37-residue LeuA and can be utilized in the synthesis of LeuA to perform structure-activity relationship (SAR) studies. Three analogues (1-3) of LeuA were designed and synthesized to explore the SAR in the N-terminal domain of LeuA. In the analogues, N-terminal -sheet residues Cys9-Ser15 of the native peptide were replaced with shorter -turn motifs. Such replacement abolished the antimicrobial activity in all the analogues. Circular dichroism spectroscopy suggested that only analogue 1 adopted similar folding as LeuA. Therefore, 1 was able to competitively inhibit the activity of native LeuA. However, analysis of the secondary structure of 1 using molecular dynamics simulations suggested lack of -sheet formation in the N-terminal region compared to LeuA emphasizing the role of proper folding and sequence in the activity of class IIa bacteriocins.
Language
English
DOI
doi:10.7939/R3J707
Rights
License granted by Krishna Bodapati (krishnac@ualberta.ca) on 2011-09-01T06:14:36Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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