Download the full-sized PDF of Temperature-Controlled Assembly Kinetics of DNA-Functionalized Gold NanoparticlesDownload the full-sized PDF



Permanent link (DOI):


Export to: EndNote  |  Zotero  |  Mendeley


This file is in the following communities:

Graduate Studies and Research, Faculty of


This file is in the following collections:

Theses and Dissertations

Temperature-Controlled Assembly Kinetics of DNA-Functionalized Gold Nanoparticles Open Access


Other title
gold nanoparticles
Type of item
Degree grantor
University of Alberta
Author or creator
Dever, Brittany R
Supervisor and department
Le, X. Chris (Chemistry, Laboratory Medicine and Pathology)
Examining committee member and department
Lucy, Charles (Chemistry)
Cairo, Christopher (Chemistry)
Campbell, Robert (Chemistry)
Li, Yingfu (Biochemistry, Chemistry)
Department of Chemistry

Date accepted
Graduation date
Doctor of Philosophy
Degree level
The assembly of nanoparticle building blocks into more complex nanostructures has important applications in analytical chemistry, materials science, medical science, optics, and electronics. Due to its exquisite specificity and programmable nature, DNA has emerged as a very promising biomolecule to facilitate the assembly of nanomaterials. DNA-conjugated gold nanoparticles (AuNP) have been used in a wide range of assays and have also been assembled into discrete nanostructures, two-dimensional arrays, and three-dimensional crystal structures. This thesis focuses on how temperature impacts the assembly kinetics of DNA-functionalized AuNPs and applying the findings to the development of analytical assays. A temperature effect on the assembly kinetics of AuNPs was discovered. As the temperature increased, the assembly kinetics increased up to a critical temperature (Tcrit). At temperatures higher than Tcrit, the assembly kinetics were drastically reduced. This very sharp transition from maximal assembly kinetics at Tcrit to minimal assembly kinetics at higher temperatures (2-3 °C) has not been reported in literature. The impact of various experimental parameters on the Tcrit¬ and the sharpness of the transition in the temperature-dependent assembly kinetics (TDAK) profile were studied. Parameters studied included the sodium chloride concentration, the amount of DNA on the AuNP surface, the linker concentration, and the presence of gaps and overhangs present in the linker sequence. The temperature dependent assembly kinetic phenomenon was used to design a colorimetric assay that could detect single nucleotide polymorphisms (SNPs) within five minutes. This strategy was used to detect a SNP that conferred first line drug resistance in Mycobacterium tuberculosis. Temperature-dependent assembly kinetics was also applied to develop a strategy to sequentially control the assembly of three different DNA-AuNPs in a single solution. These results demonstrate the broad applications that temperature-dependent assembly kinetics of DNA-AuNPs can have in assay development and the synthesis of more complex nanostructures from nanoparticle building blocks. With the promising applications that nanotechnology has to offer, temperature-dependent assembly kinetics offers a novel tool to selectively assemble nanomaterials in solution to aid in the advancement of nanoscience.
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
Citation for previous publication
Zhang, H., Li, F., Dever, B., Li, X.-F., Le, X.C. DNA-mediated homogeneous binding assays for nucleic acids and proteins. Chemical Reviews, 113, 2812.

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
File format: pdf (PDF/A)
Mime type: application/pdf
File size: 5312666
Last modified: 2016:11:16 16:22:42-07:00
Filename: Dever_Brittany_R_201409_PhD.pdf
Original checksum: c4fa0dd8c1c7adc1c4984d432f16f95d
Activity of users you follow
User Activity Date