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Permanent link (DOI): https://doi.org/10.7939/R3KH0F45W

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Approaches to Reverse Red Cell Sickling Open Access

Descriptions

Other title
Subject/Keyword
Reversing
Hemoglobin S
Anthocyanin
Sickle
delphinidin
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Basonbul, Asmaa A
Supervisor and department
Ms. Lew, Kristi (Laboratory Medicine and Pathology)
Dr. Acker, Jason (Laboratory Medicine and Pathology)
Examining committee member and department
Dr. Estey, Mathew (Laboratory Medicine and Pathology)
Dr. Onell, Rodrigo (Laboratory Medicine and Pathology)
Department
Medical Sciences-Laboratory Medicine and Pathology
Specialization

Date accepted
2016-03-08T13:23:21Z
Graduation date
2016-06
Degree
Master of Science
Degree level
Master's
Abstract
There is evidence that the symptoms associated with sickle cell disease can be relieved by using plant extracts containing anthocyanin (AC) agents. It has been established that ACs were able to reverse sickle cell morphology to a biconcave disc shape. However, no specific form of AC has been investigated in detail. In this thesis, a purified manufactured compound, delphinidin, was selected for study due to its antioxidant property. This study hypothesized that delphinidin chloride (Delph-CL) can reverse the sickle cell morphology by inhibiting hemoglobin S (Hb S) polymerization through a hydration and/or antioxidation biomechanism. Furthermore, the effect of Delph-CL on membrane integrity, Hb S solubility, and O2 binding were evaluated. Exposure to 100 µɡ/mL Delph-CL caused some sickle cells to transform into an elliptical shape (P = 0.04), possibly as a transition stage to discoid cells. Hb S polymerization also decreased which resulted in more O2 binding and a low P50. However, Delph-CL acted negatively on the red cell membrane by causing hemolysis. Neither one of the proposed biomechanisms were achieved and further work is necessary to establish the mechanism of sickle cell reversal.
Language
English
DOI
doi:10.7939/R3KH0F45W
Rights
This thesis is made available by the University of Alberta Libraries with permission of the copyright owner solely for the purpose of private, scholarly or scientific research. This thesis, or any portion thereof, may not otherwise be copied or reproduced without the written consent of the copyright owner, except to the extent permitted by Canadian copyright law.
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