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Permanent link (DOI): https://doi.org/10.7939/R36W96P4G

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Repeated oronasal exposure to lipopolysaccharide induced mucosal IgA responses in periparturient dairy cows Open Access

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Author or creator
Iqbal, Summera
Zebeli, Qendrim
Mansmann, Dominik A.
Dunn, Suzanna M.
Ametaj, Burim N.
Additional contributors
Subject/Keyword
Mucus
Antibodies
Haptoglobins
Saline Solutions
Enzyme-Linked Immunoassays
Immune Response
Blood Plasma
Saliva
Type of item
Journal Article (Published)
Language
English
Place
Time
Description
This study investigated the effects of repeated oronasal treatment with lipopolysaccharide (LPS) on the humoral immune responses in saliva, vaginal mucus, and the plasma markers of the acute phase response in periparturient dairy cows. One hundred pregnant Holstein cows were administered either 3 increasing doses of LPS (n = 50) as follows: 1) 0.01 µg/kg body weight (BW) on d −28, 2) 0.05 µg/kg BW on d −25, and −21, and 3) 0.1 µg/kg BW on d −18, and −14, or sterile saline solution (controls; n = 50) oronasally for 3 consecutive wk starting at 28 d before parturition. Intensive sampling was conducted on thirty cows (n = 15/group). Multiple saliva, vaginal mucus and blood samples were collected around parturition and analyzed for total immunoglobulin-(Ig)A, plasma serum amyloid A (SAA), lipopolysaccharide-binding protein (LBP), anti-LPS IgA, IgG, IgM, tumour necrosis factor(TNF)-α, and interleukin(IL)-1. Results regarding total secretory IgA (sIgA) antibodies showed greater concentrations in the saliva and an overall tendency for higher total sIgA in the vaginal mucus of the LPS-treated cows. Treatment had no effect on plasma sIgA, IgG, IgM anti-LPS antibodies, haptoglobin, SAA, LBP, TNF-α, and IL-1. Treatments by time interactions were observed for SAA and IL-1 with lowered concentrations of both variables in the plasma of LPS-treated cows after parturition. Overall, repeated oronasal LPS treatment clearly enhanced total sIgA antibodies in the saliva, stimulated their production in vaginal mucus shortly before calving, and lowered plasma IL-1 around parturition, but showed limited effects on markers of the acute phase response in the plasma in dairy cows around parturition.
Date created
2014
DOI
doi:10.7939/R36W96P4G
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Attribution 4.0 International
Citation for previous publication
Iqbal, S., Zebeli, Q., Mansmann, D. A., Dunn, S. M., & Ametaj, B. N. (2014). Repeated oronasal exposure to lipopolysaccharide induced mucosal IgA responses in periparturient dairy cows. PLoS ONE, 9(7), e103504 [10 pages].  http://dx.doi.org/10.1371/journal.pone.0103504

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