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Investigation of the Kinetics of Tet(O)-mediated Tetracycline Resistance Open Access


Other title
Tet(O), tetracycline resistance, kinetics
Type of item
Degree grantor
University of Alberta
Author or creator
Li, Jun
Supervisor and department
Monika Keelan (Laboratory Medicine and Pathology)
Examining committee member and department
Michael Gänzle (Agricultural, Food and Nutritional Sciences)
Richard Fahlman (Biochemistry)
Jeff Fuller (Laboratory Medicine and Pathology)
Medical Sciences - Laboratory Medicine and Pathology

Date accepted
Graduation date
Master of Science
Degree level
Widespread tetracycline resistance (TcR) has limited the clinical use of Tc for the treatment of bacterial infections. Tet(O) protein is present in many bacteria and is the major transmissible TcR determinant in Campylobacter jejuni, a common cause of acute bacterial diarrhea worldwide. Tet(O) protects ribosomes against the inhibition of protein synthesis by Tc. Tet(O) binds to the ribosome at a similar site as EF-G, a structural homologue of Tet(O) with GTPase activity that is required for protein elongation. EF-G interfered with the kinetics of Tet(O)-mediated Tc release suggesting that EF-G competes with Tet(O) for ribosome binding. Indirect assessment of EF-G and Tet(O) binding to 70S ribosomes by GTP hydrolysis was unable to clearly demonstrate competition for binding. This thesis contributed to the further understanding of the kinetics of Tc release by Tet(O), and may facilitate the development of novel strategies to overcome Tet(O)-mediated TcR in bacteria which cause human infections.
License granted by Jun Li ( on 2010-04-15T22:36:25Z (GMT): Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of the above terms. The author reserves all other publication and other rights in association with the copyright in the thesis, and except as herein provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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