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Functional Imaging of Cancer Mitochondria with Multiphoton Confocal Microscopy Open Access


Other title
TMRM and Mitosox
Dichloroacetate DCA
Confocal Microscopy
Glioblastoma Multiforme
Cancer Metabolic Modulators
Type of item
Degree grantor
University of Alberta
Author or creator
Haromy, Alois S
Supervisor and department
Michelakis, Evangelos (Medicine)
McMurtry, Sean (Medicine)
Examining committee member and department
Michelakis, Evangelos (Medicine)
Tsuyuki, Ross (Medicine)
Tsui, Ying Yin (Department of Electrical and Computer Engineering)
McMurtry, Sean (Medicine)
Department of Medicine
Experimental Medicine
Date accepted
Graduation date
Master of Science
Degree level
Maximizing the possibility of effectively treating cancer requires timely assessment of its aggressiveness and susceptibility to drug intervention. During cancer surgery, the “fast biopsy” is a commonly employed technique to assess a tumor’s invasiveness and guide surgery. Unfortunately, it is of limited value in predicting a tumor’s metastatic potential and susceptibility to drug treatment. However, certain functional biomarkers, like mitochondrial membrane potential and reactive oxygen species, which may be indicative of both aggressiveness and drug susceptibility, can be measured in “real time” using confocal microscopy. To this end, theoretical and practical aspects of functional confocal imaging are presented as a general overview and in conjunction with a clinical trial treating glioblastoma multiforme (GBM) using the metabolic modulating drug DCA. Functional imaging of biopsies from 40 GBM patients, various cancer and normal cell lines validated confocal microscopy as a tool assessing functional biomarkers and the effectiveness of DCA treatment.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
E. D. Michelakis et al., Sci Transl Med 2, 31ra34 (May 12, 2010).

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