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Quantification of bacteriocin gene expression in Carnobacterium maltaromaticum ATCC PTA-5313 Open Access


Other title
gene expression
Type of item
Degree grantor
University of Alberta
Author or creator
Balutis, Andrea M
Supervisor and department
McMullen, Lynn (Agricultural, Food and Nutritional Science)
Examining committee member and department
McMullen, Lynn (Agricultural, Food and Nutritional Science)
Gänzle, Michael (Agricultural, Food and Nutritional Science)
Jeon,Byeong Hwa (School of Public Health)
Department of Agricultural, Food, and Nutritional Science
Food Science and Technology
Date accepted
Graduation date
Master of Science
Degree level
Carnobacterium maltaromaticum ATCC PTA-5313, which produces bacteriocins carnocyclin A, piscicolin 126 and carnobacteriocin BM1, has been approved for use on meat products to control the growth of Listeria monocytogenes. This combination of bacteriocins is very effective as a biopreservative. It was unclear which of the three bacteriocins is responsible for the antimicrobial effect on meat. The aim of this research was to develop a novel method for detection of bacteriocin gene expression and to use this method to determine the expression of bacteriocin genes when C. maltaromaticum was inoculated onto a ready-to-eat meat product. A novel method to detect bacteriocin gene expression in vitro and on a low sodium ready-to-eat meat product was developed using a modified RNA extraction protocol and quantitative polymerase chain reaction. When C. maltaromaticum ATCC PTA-5313 was grown in broth in the presence of lactate and acetate preservatives, there was no change in gene expression during growth. When C. maltaromaticum ATCC PTA-5313 was inoculated onto ham and stored for 28 days at 4C, the structural genes for all three bacteriocins were expressed. During storage, gene expression decreased relative to a culture grown in broth at the same temperature. Gene expression was monitored every 4 to 7 days up to 56 days of storage on vacuum packaged low sodium ham formulated with or without preservatives and inoculated with C. maltaromaticum ATCC PTA-5313 and a 5-strain cocktail of L. monocytogenes. There was an increase in expression of the piscicolin 126 and carnobacteriocin BM1 genes during the stationary phase of growth of the cultures on ham formulated with preservatives. Overall, bacteriocin gene expression from C. maltaromaticum ATCC PTA-5313 is decreased over storage time when inoculated on vacuum packaged ham, and the presence of preservatives results in higher expression levels during stationary phase than that of C. maltaromaticum ATCC PTA-5313 on ham without preservatives.
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