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University of Alberta Celebration of Research and Innovation


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2010 2nd Annual Celebration of Research and Innovation

Myosin-driven peroxisome partitioning in S. cerevisiae. Open Access


Author or creator
Fagarasanu, A.
Mast, F.D.
Knoblach, B.
Jin, Y.
Brunner, M.J.
Logan, M.R.
Glover, J.N.M.
Eitzen, G.A.
Atichison, J.D.
Weisman, L.S.
Rachubinski, R.A.
Additional contributors
Myosin Heavy Chains/metabolism
Saccharomyces cerevisiae Proteins/metabolism
Saccharomyces cerevisiae/cytology
Myosin Type V/metabolism
cell cycle/physiology
Type of item
Journal Article (Published)
In Saccharomyces cerevisiae, the class V myosin motor Myo2p propels the movement of most organelles. We recently identified Inp2p as the peroxisome-specific receptor for Myo2p. In this study, we delineate the region of Myo2p devoted to binding peroxisomes. Using mutants of Myo2p specifically impaired in peroxisome binding, we dissect cell cycle–dependent and peroxisome partitioning–dependent mechanisms of Inp2p regulation. We find that although total Inp2p levels oscillate with the cell cycle, Inp2p levels on individual peroxisomes are controlled by peroxisome inheritance, as Inp2p aberrantly accumulates and decorates all peroxisomes in mother cells when peroxisome partitioning is abolished. We also find that Inp2p is a phosphoprotein whose level of phosphorylation is coupled to the cell cycle irrespective of peroxisome positioning in the cell. Our findings demonstrate that both organelle positioning and cell cycle progression control the levels of organelle-specific receptors for molecular motors to ultimately achieve an equidistribution of compartments between mother and daughter cells.
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Creative Commons Attribution-Non-Commercial-No Derivatives 3.0 Unported

Citation for previous publication
Fagarasanu, A., F.D. Mast, B. Knoblach, Y. Jin, M.J. Brunner, M.R. Logan, J.N.M. Glover, G.A. Eitzen, J.D. Aitchison, L.S. Weisman, and R.A. Rachubinski. 2009. Myosin-driven peroxisome partitioning in S. cerevisiae. J Cell Biol.186:541-554.
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