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Permanent link (DOI): https://doi.org/10.7939/R30R9MB0S
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Endothelial Colony Forming Cells: Role in Bronchopulmonary Dysplasia and evaluation of new therapeutic strategies Open Access
- Other title
Endothelial Colony Forming Cells
- Type of item
- Degree grantor
University of Alberta
- Author or creator
Rajabali, Saima Nasiruddin
- Supervisor and department
Dr. Bernard Thebaud, Department of Pediatrics
- Examining committee member and department
Dr. Gary Lopaschuk, Department of Pediatrics
Dr. John Greer, Department of Physiology
Dr. Jason Dyck, Department of Pediatrics
- Date accepted
- Graduation date
Master of Science
- Degree level
Bronchopulmonary dysplasia (BPD), a chronic lung disease of prematurity, results in alveolar simplification and respiratory distress in the newborn. Vascular component is implicated. Lung damage involves a deficiency in the number and function of progenitor cells.
We hypothesize that endothelial colony forming cells (ECFCs) exist in the developing human lung, are impaired in hyperoxia and secrete exosomes.
ECFCs from human fetal lung expressed CD31, CD105, CD144, CD146 and were negative for CD14 and CD45. In hyperoxic conditions, cord formation and clonogenic potential was impaired. Mesenchymal stem cell conditioned media (MSC CdM) improved clonogenic potential. Exosomes were isolated from human cord blood derived ECFC CdM and characterized using electron microscopy and protein expression.
This study provides novel finding that ECFCs exist in human fetal lung and their function is impaired in hyperoxia. They may exert their effect by exosomes. This provides a rationale for use of exogenous stem cells in BPD.
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- Citation for previous publication
The Emerging Role of Stem/Progenitor Cells in Pulmonary Vascular Disease. Saima Rajabali, Duncan J. Stewart, Shirley H. J. Mei and Bernard Thébaud. J Clin Exp Cardiolog 2012, S8-004
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