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Permanent link (DOI): https://doi.org/10.7939/R30R9MB0S

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Endothelial Colony Forming Cells: Role in Bronchopulmonary Dysplasia and evaluation of new therapeutic strategies Open Access

Descriptions

Other title
Subject/Keyword
Exosomes
Bronchopulmonary Dysplasia
Stem cells
Endothelial Colony Forming Cells
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Rajabali, Saima Nasiruddin
Supervisor and department
Dr. Bernard Thebaud, Department of Pediatrics
Examining committee member and department
Dr. Gary Lopaschuk, Department of Pediatrics
Dr. John Greer, Department of Physiology
Dr. Jason Dyck, Department of Pediatrics
Department
Medical Sciences-Paediatrics
Specialization

Date accepted
2013-09-26T11:13:09Z
Graduation date
2013-11
Degree
Master of Science
Degree level
Master's
Abstract
Bronchopulmonary dysplasia (BPD), a chronic lung disease of prematurity, results in alveolar simplification and respiratory distress in the newborn. Vascular component is implicated. Lung damage involves a deficiency in the number and function of progenitor cells. We hypothesize that endothelial colony forming cells (ECFCs) exist in the developing human lung, are impaired in hyperoxia and secrete exosomes. ECFCs from human fetal lung expressed CD31, CD105, CD144, CD146 and were negative for CD14 and CD45. In hyperoxic conditions, cord formation and clonogenic potential was impaired. Mesenchymal stem cell conditioned media (MSC CdM) improved clonogenic potential. Exosomes were isolated from human cord blood derived ECFC CdM and characterized using electron microscopy and protein expression. This study provides novel finding that ECFCs exist in human fetal lung and their function is impaired in hyperoxia. They may exert their effect by exosomes. This provides a rationale for use of exogenous stem cells in BPD.
Language
English
DOI
doi:10.7939/R30R9MB0S
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
The Emerging Role of Stem/Progenitor Cells in Pulmonary Vascular Disease. Saima Rajabali, Duncan J. Stewart, Shirley H. J. Mei and Bernard Thébaud. J Clin Exp Cardiolog 2012, S8-004

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File title: Dedication
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Page count: 124
File language: en-CA
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