ERA

Download the full-sized PDF of Characterization of the insect specific putative kinase banshee in Drosophila melanogasterDownload the full-sized PDF

Analytics

Share

Permanent link (DOI): https://doi.org/10.7939/R3X921S57

Download

Export to: EndNote  |  Zotero  |  Mendeley

Communities

This file is in the following communities:

Graduate Studies and Research, Faculty of

Collections

This file is in the following collections:

Theses and Dissertations

Characterization of the insect specific putative kinase banshee in Drosophila melanogaster Open Access

Descriptions

Other title
Subject/Keyword
Molecular Genetics
Enhancer of variegation
Mutant analysis
Antibody optimization
P Element Dependant Silencing
Drosophila melanogaster
Insect Specific
Kinase
Type of item
Thesis
Degree grantor
University of Alberta
Author or creator
Canham, Lindsay C
Supervisor and department
Locke, John (Biological Sciences)
Examining committee member and department
Campbell, Shelagh (Biological Sciences)
Hughes, Sarah (Medical Genetics)
Department
Department of Biological Sciences
Specialization
Molecular Biology and Genetics
Date accepted
2015-09-22T11:10:59Z
Graduation date
2015-11
Degree
Master of Science
Degree level
Master's
Abstract
In differentiating cells, genes are silenced or transcribed through changes in chromatin organization. Active chromatin is known as euchromatin and repressive chromatin is known as heterochromatin. These active or repressive states are initiated and maintained by modifying residues of the core histone tails. Previous work has identified Su(var) and E(var) genes as modifiers of histone, with some roles in initiating heterochromatin or maintaining euchromatin, respectively. The gene bshe (CG8878) was identified in a forward genetic screen looking for recessive lethal Drosophila E(var) mutants. The amino acid sequence of this previously unstudied gene is predicted to be a protein kinase, based on its similarity to a known histone kinase. My research has begun to explore the function of bshe, and how it contributes to the heterochromatin/euchromatin balance. The predicted BSHE polypeptide has a kinase domain within the sequence. However, it also has a large interruption in the middle of the catalytic regions of the kinase domain, calling in to question whether it truly has kinase activity. Through phylogenetic analysis I characterize bshe to be an insect specific kinase of either an ancient or rapidly evolving clade. Predictions of the protein structure suggest that despite the large interruption, the main catalytic regions of the kinase domain are still in correct confirmation, suggesting that it still functions as a kinase. My observations of bshe mutant phenotypes show the majority of mutant hemizygotes to die by second instar larvae, with a maternal effect leading to earlier lethality. I completed an initial optimization of three antibodies, made against three polypeptides from BSHE, for Western blots and immunofluorescence. Initial observations show in syncytial embryos BSHE is localized to the cytoplasm.
Language
English
DOI
doi:10.7939/R3X921S57
Rights
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication

File Details

Date Uploaded
Date Modified
2015-09-22T17:11:00.527+00:00
Audit Status
Audits have not yet been run on this file.
Characterization
File format: pdf (PDF/A)
Mime type: application/pdf
File size: 4756852
Last modified: 2016:06:24 17:46:58-06:00
Filename: Canham_Lindsay_C_201509_MSc.pdf
Original checksum: 914bef4ec145f87b394704fbd2b83e21
Activity of users you follow
User Activity Date