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Bladder outlet obstruction: progression from inflammation to fibrosis Open Access


Other title
Bladder outlet obstruction
Type of item
Degree grantor
University of Alberta
Author or creator
Metcalfe, Peter
Supervisor and department
Tredget, Edward (Surgery)
Moore, Ron (Surgery)
Burrell, Robert (Biomedical Engineering)
Examining committee member and department
Burrell, Robert (Biomedical Engineering)
Tredget, Ted (Surgery)
Moore, Ron (Surgery)
Department of Surgery

Date accepted
Graduation date
Master of Science
Degree level
Abstract: Introduction: Partial bladder outlet obstruction (pBOO) is a ubiquitous problem that results in renal damage. We hypothesize that change in the bladder progresses over time. Methods: Fischer rats underwent surgical pBOO for 2, 4, 8, or 13 weeks and were compared to shams. Urodynamic measurements were taken, bladders weight and thickness recorded, and tissue analyzed with microscopy. RT-PCR was performed for inflammatory mediators and spectrometry used to quantify collagen. Results: Urodynamics demonstrated an increased capacity and deterioration into high-pressure. H+E demonstrated an initial inflammatory response, and increased mRNA levels of TGF-β, CTGF, HIF-1α, and PDGF. Muscle hypertrophy was evident on H+E and increased bladder mass and thickness. Masson’s Trichrome and mass spectrometry showed an increase in collagen. Conclusion: We believe that this represents distinct phases of bladder decompensation: inflammation, hypertrophy, and fibrosis. This could lead to improved preventative strategies, with respect to biochemical pathways and the time course of their initiation.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
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File title: Thesis June 17 2010
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