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The Cpx pathway causes changes in peptidoglycan structure, turnover, and recycling Open Access


Other title
Cpx pathway
Cell wall
E. coli
stress responses
Lytic transglycosylase
Type of item
Degree grantor
University of Alberta
Author or creator
Bernal, María Margarita
Supervisor and department
Raivio, Tracy (Biological Sciences)
Examining committee member and department
Stein, Lisa (Biological Sciences)
Szymanski, Christine (Biological Sciences)
Owttrim, George (Biological Sciences)
Department of Biological Sciences
Microbiology and Biotechnology
Date accepted
Graduation date
Master of Science
Degree level
Bacteria need to adapt to the ever-changing conditions in the environment and Escherichia coli employs the Cpx two-component system to protect the envelope, which consists of the inner membrane, periplasm, peptidoglycan, and outer membrane. Cpx is known to sense and respond to protein misfolding in the periplasm and changes to the inner membrane. In this study, we investigate the effect of the activation of the Cpx pathway on the peptidoglycan (PG) structure. We confirm the CpxR-mediated regulation of amiA, amiC, dacC, slt, mltB, ygaU, and ycbB using luminescent reporters. In addition, we confirm that these genes are activated in response to Cpx-inducing conditions. HPLC analysis shows that activation of four genes – ycbB, ygaU, slt70 and mltB - leads to changes in peptidoglycan cross-linking and turn over. Furthermore, absence of ycbB and ygaU caused activation of the Cpx pathway, suggesting that changes in PG can induce this stress response.
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