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Permanent link (DOI): https://doi.org/10.7939/R3794177K

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Eunapius fragilis Trinity transcriptome Open Access

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Author or creator
Sally Leys
Additional contributors
Subject/Keyword
Freshwater sponge
Porifera
Sponge
Transcriptome
Type of item
Research Material
Language
English
Place
Time
Description
ABSTRACT Background: The Wnt signaling pathway is uniquely metazoan and used in many processes during development, including the formation of polarity and body axes. In sponges, one of the earliest diverging animal groups, Wnt pathway genes have diverse expression patterns in different groups including along the anterior-posterior axis of two sponge larvae, and in the osculum and ostia of others. We studied the function of Wnt signaling and body polarity formation through expression, knockdown, and larval manipulation in several freshwater sponge species. Results: Sponge Wnts fall into sponge-specific and sponge-class specific subfamilies of Wnt proteins. Notably Wnt genes were not found in transcriptomes of the glass sponge Aphrocallistes vastus. Wnt and its signaling genes were expressed in archaeocytes of the mesohyl throughout developing freshwater sponges. Osculum formation was enhanced by GSK3 knockdown, and Wnt antagonists inhibited both osculum development and regeneration. Using dye tracking we found that the posterior poles of freshwater sponge larvae give rise to tissue that will form the osculum following metamorphosis. Conclusions: Together the data indicate that while components of canonical Wnt signaling may be used in development and maintenance of osculum tissue, it is likely that Wnt signaling itself occurs between individual cells rather than whole tissues or structures in freshwater sponges.
Date created
2017/11/08
DOI
doi:10.7939/R3794177K
License information
Attribution-NonCommercial-NoDerivs 4.0 International
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Citation for previous publication
Windsor et al. submitted to BMC Evolutionary Biology November 2017
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