Download the full-sized PDF of The endosymbiont Wolbachia pipientis induces the expression of host antioxidant proteins in an Aedes albopictus cell lineDownload the full-sized PDF



Permanent link (DOI):


Export to: EndNote  |  Zotero  |  Mendeley


This file is in the following communities:

Biological Sciences, Department of


This file is in the following collections:

Journal Articles (Biological Sciences)

The endosymbiont Wolbachia pipientis induces the expression of host antioxidant proteins in an Aedes albopictus cell line Open Access


Author or creator
Brennan, L.J.
Keddie, B.A.
Braig, H.R.
Harris, H.L.
Additional contributors
antioxidant proteins
reactive oxygen species
antimicrobial peptides
immune responses
Type of item
Journal Article (Published)
Wolbachia are obligate intracellular bacteria which commonly infect arthropods. They are maternally inherited and capable of altering host development, sex determination, and reproduction. Reproductive manipulations include feminization, male-killing, parthenogenesis, and cytoplasmic incompatibility. The mechanism by which Wolbachia avoid destruction by the host immune response is unknown. Generation of antimicrobial peptides (AMPs) and reactive oxygen species (ROS) by the host are among the first lines of traditional antimicrobial defense. Previous work shows no link between a Wolbachia infection and the induction of AMPs. Here we compare the expression of protein in a cell line naturally infected with Wolbachia and an identical cell line cured of the infection through the use of antibiotics. Protein extracts of each cell line were analyzed by two dimensional gel electrophoresis and LC/MS/MS. Our results show the upregulation of host antioxidant proteins, which are active against ROS generated by aerobic cell metabolism and during an immune response. Furthermore, flow cytometric and microscopic analysis demonstrates that ROS production is significantly greater in Wolbachia-infected mosquito cells and is associated with endosymbiont-containing vacuoles located in the host cell cytoplasm. This is the first empirical data supporting an association between Wolbachia and the insect antioxidant system.
Date created
License information
© 2008 Brennan et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Citation for previous publication
.Brennan LJ, Keddie BA, Braig HR, Harris HL (2008) The Endosymbiont Wolbachia pipientis Induces the Expression of Host Antioxidant Proteins in an Aedes albopictus Cell Line. PLoS ONE 3(5): e2083. doi:10.1371/journal.pone.0002083
Link to related item

File Details

Date Uploaded
Date Modified
Audit Status
Audits have not yet been run on this file.
File format: pdf (PDF/A)
Mime type: application/pdf
File size: 301915
Last modified: 2015:10:12 18:09:34-06:00
Filename: PLOS_ONE_3_2008_e2083.pdf
Original checksum: b9373009ba45ec1fe83526cea600cea2
Well formed: true
Valid: true
File title: pone.0002083 1..8
Page count: 8
Activity of users you follow
User Activity Date