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Quantitative trait loci (QTL) mapping and doubled haploid technology for spring wheat improvement Open Access


Other title
Quantitative trait loci
doubled haploid
Type of item
Degree grantor
University of Alberta
Author or creator
Asif, Muhammad
Supervisor and department
Spaner, Dean
Examining committee member and department
Rahman, Habibur
Randhawa, Harpinder
Strelkov, Stephan
Yang, Rong-Cai
Singh, Jaswinder
Department of Agricultural, Food, and Nutritional Science
Plant Science
Date accepted
Graduation date
Doctor of Philosophy
Degree level
Efficiency is important in wheat breeding programs. A series of studies were conducted to determine genetic differentials, map genomic regions affecting various agronomic and quality traits in conventional and organic management systems and to improve doubled haploid (DH) technology in spring wheat. A mapping population developed from a cross between Attila x CDC Go performed differently in the two management systems. Heritability estimates differed between systems for five of the nine traits including grain yield, tillers, plant height, kernel weight and grain protein content. Direct selection in each management system resulted in 50% or less lines selected in common for eight of the nine (except for flowering time) traits. Overall, we mapped 46 QTL for various agronomic and quality traits across organic and conventional management systems for three years. Most of these QTL were specific to the management systems; however, consistent QTL for grain yield, test weight, kernel weight and days to flowering were mapped in both systems on chromosomes 6A, 1B, 3A and 5B, respectively. These consistent QTL also behaved differently in both systems with respect to their phenotypic variation and additive effects leading to the conclusion that selection and testing of genotypes for production and quality traits should be done within environmental conditions which are comparable to where candidate lines are intended to perform. The technique of isolated microspore culture (IMC) is being used in many species to produce DH plants; however, low frequency of green plant production, microbial contamination and albinism limit its use in wheat breeding programs. A series of experiments were conducted to improve microspore embryogenesis. In four different experiments, induction medium (NPB-99+10% Ficoll) was supplemented with various organelle antioxidants, antibiotics and phytosulfokine alpha (PSK-α) along with optimization of induction medium osmolality. Glutathione (2 µM), PSK-α (10-7 M) and osmolality of induction medium at 350 mOsm kg-1 enhanced the efficiency of microspore embryogenesis in wheat and triticale. Cefotaxime at 100 and 50 mg L-1 also helped to control recurrent bacterial contamination in our laboratory, reduce the occurrence of albinism and substantially increase the formation of embryo/embryo like structures and green plant production in wheat and triticale, respectively. The systematic use of these treatments in IMC of wheat and triticale is recommended.
Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission.
Citation for previous publication
Randhawa HS, Asif M, Pozniak C, Clarke JM, Graf RJ, Fox SL, Humphreys DG, Knox RE, DePauw RM, Singh AK, Cuthbert RD, Hucl P, Spaner D (2013) Application of molecular markers to wheat breeding in Canada. Plant Breeding. DOI: 10.1111/pbr.12057Asif M, Eudes F, Goyal A, Amundsen E, Randhawa HS, Spaner D (2013) Organelle antioxidants improve microspore embryogenesis in wheat and triticale. In Vitro Cellular and Developmental Biology - Plant. 49:489-497Asif M, Eudes F, Randhawa HS, Amundsen E, Spaner D (2014) Induction medium osmolality improves microspore embryogenesis in wheat and triticale. In Vitro Cellular and Developmental Biology - Plant. 50:121-126Asif M, Eudes F, Randhawa HS, Amundsen E, Yanke J, Spaner D (2013) Cefotaxime prevents microbial contamination and improves microspore embryogenesis in wheat and triticale. Plant Cell Reports. 32:1637-1646Asif M, Eudes F, Randhawa HS, Amundsen E, Spaner D (2014) Phytosulfokine alpha enhances microspore embryogenesis in both triticale and wheat. Plant Cell Tissue and Organ Culture. 116:125-130Asif, M. (2013) Androgenesis: a fascinating doubled haploid production process. In: Asif, M. Progress and opportunities of doubled haploid production. Springer, New York. pp:7-44

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