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- 2Electrospray ionization
- 2Library screening
- 2Protein-carbohydrate interactions
- 1Carbohydrate-Protein interactions
Results for "supervisors_tesim:"Klassen, John (Department of chemistry)""
Quantitative Characterization of Noncovalent Protein-Carbohydrate Interactions using Electrospray Ionization Mass SpectrometryDownload
The interactions between water-soluble proteins and carbohydrates found on the surfaces of cells play important roles in many physiological and pathological cellular processes. Carbohydrates function as receptors for signaling, cellular recognition and adhesion, and pathogen infections. This...
This thesis describes the application of mass spectrometry (MS) to glycoprotein and oligosaccharide analysis. Glycosylated proteins are involved in cell-cell and cell-matrix recognition. Applications of trypsin and proteinase K to hydrolyze glycoproteins into glycopeptides that are compatible...
Development and Application of ESI-MS Based Techniques for Quantitative Determination of Protein-Carbohydrate InteractionsDownload
This thesis describes the development and application of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was employed to quantify protein-carbohydrate interactions in aqueous buffer...
Study of Non-Covalent Protein-Carbohydrate Interactions using Electrospray Ionization Mass SpectrometryDownload
This thesis describes the development and application of electrospray ionization mass spectrometry (ESI-MS) methods to study protein-carbohydrate interactions in vitro. The affinities (Ka) of two recombinant fragments of Clostridium difficile toxins (TcdA & TcdB) and a library of the most...