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- 1Asymmetric pulsed field
- 1Bead packing
- 1Colloidal crystals
- 1Colloidal self-assembly
- 1DNA Trapping
Nanomaterials have been widely involved in biomolecular analysis, to refine traditional analytical method or develop complementary techniques. In this thesis, nanoporous structures were utilized in two applications, DNA electrophoresis and solid matrix laser desorption ionization (SMALDI)....
This dissertation investigates the size based separation of DNA molecules in nanoparticle arrays under asymmetric pulsed electrophoresis. Crystalline arrays of nanoparticles within microfluidic channels are fabricated using colloidal self-assembly, yielding structures with pore sizes ranging from...
Interest in microfluidic platforms has surged as an alternative for sample preparation in the past two decades, with the potential for miniaturization, portability, automation, integration and parallelism driving this research. However, it is still very challenging to develop an integrated...
Directed Evolution Approaches for Improved Genetically Encoded Fluorescent Calcium Ion and Voltage IndicatorsDownload
Fluorescent proteins (FP) have revolutionized our imaging technologies and facilitated visualization of biochemical and physiological processes occurring in complex biological systems, which has opened up a new and unprecedented era in cell biology. Although FPs mainly serve as passive...
The last decades witnessed the rapid growth and advancement of research on microfluidic systems for chemical and biological analysis. There remain many important biochemical challenges that need to be solved with novel technologies. This thesis looks at how to apply several microfluidic...
This thesis reports the use of high magnitude asymmetric pulsed fields and high frequency zero-field interruptions on the pulses to accomplish angular separation of megabase and submegabase sized DNA in colloidal self-assembled silica particle arrays. First, a simple particle array packing...
DNA strand displacement reaction (SDR) is a fast, isothermal, and sequence specific reaction. In SDR, an invading DNA single strand, also known as the “fuel” strand, unzips a partial duplex to form a more thermodynamically stable duplex. The complementary strand to the fuel strand is referred to...
This thesis reports a facile method to stabilize colloidal self-assembled (CSA) 310 and 50 nm nanoparticles packed in microchannels for high speed size-based separation of denatured proteins. Silica nanoparticles, self-assembled in a network of microfluidic channels, were stabilized with a...