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Skip to Search Results- 1Dalangin, Rochelin R. S.
- 1Hoi, Hiofan
- 1Lai, Yan Tiffany
- 1Lu, Xiaocen
- 1Saini, Ritesh K.
- 1Wu, Sheng Yi
- 3Fluorescent proteins
- 2Directed evolution
- 1Alternate frame folding
- 1AvGFP
- 1Bimolecular fluorescence complementation
- 1Biosensors
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Spring 2013
Fluorescent protein (FP) technology is now an indispensible tool of biomedical research. Nevertheless, only a few members of the hundreds of existing FPs are generally regarded as the preferred options for most imaging applications. Accordingly, new FPs with ever-improved properties are in demand...
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Directed Evolution Approaches for Improved Genetically Encoded Fluorescent Calcium Ion and Voltage Indicators
DownloadFall 2014
Fluorescent proteins (FP) have revolutionized our imaging technologies and facilitated visualization of biochemical and physiological processes occurring in complex biological systems, which has opened up a new and unprecedented era in cell biology. Although FPs mainly serve as passive...
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Spring 2021
Information is transmitted between neurons through the flow of different ions and the release of neurotransmitters at speeds on the order of milliseconds. The advent of genetically encoded fluorescent protein (FP)-based optical indicators for neuronal activity, which can be precisely targeted...
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Fall 2015
The expansion of the fluorescent protein (FP) colour palette through discovery of more species possessing FPs and by subjecting them to intensive protein engineering efforts has greatly aided the effectiveness of FP-based biosensor designs. Currently, the main FP-based methods for detecting...
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Genetically encoded fluorescent biosensors for detection of ligands and protein-protein interactions
DownloadSpring 2021
Fluorescence imaging enables direct visualization of cellular activities with remarkable resolutions supported by advancing technologies. The fluorescent markers for biomolecules complement the hardware and software for imaging a specific target in detail. Genetically encoded fluorescent proteins...