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- 1Affinity chromatography
- 1Cell migration
- 1DANA derivatives
- 1Enzyme inhibitors
Protein phosphorylation is a crucial component in physiological signal transduction pathways. It is estimated that one-third of all cellular proteins are modified through phosphorylation, and these pathways are regulated by kinase and phosphatase enzymes. Glucose-6-phosphatase (G6Pase) is an...
Sialidase enzymes play an important role in regulation of cellular activities by hydrolyzing the terminal, non-reducing sialic acids attached to various glycolipids, glycoproteins, and gangliosides. The family of human sialidase enzymes, NEU1, NEU2, NEU3, and NEU4 contribute specifically to...
IMMOBILIZATION OF OLIGOSACCHARIDES IN AFFINITY CHROMATOGRAPHY AND APPLICATIONS TO SCREENING HUMAN MILK OLIGOSACCHARIDE RECEPTORSDownload
A variety of applications in glycobiology exploit affinity chromatography through the immobilization of glycans to a solid support. Although several strategies are available, in many cases there is a lack of satisfactory chemical characterization. In this thesis we describe work towards improved...
The human neuraminidases (NEU1, NEU2, NEU3, and NEU4) are a family of enzymes implicated in pathologies including cancer and diabetes. Our group has developed selective inhibitors for these enzymes that have been employed as tools to understand their biological roles. Several reports have linked...
Sialidases (neuraminidases) are a group of enzymes responsible for the hydrolysis of sialic acid from glycoconjugates. In humans, there are four different isoenzymes that play important roles in health and disease. The human neuraminidase enzymes (hNEU) were discovered somewhat recently, and...
The human neuraminidase enzyme, NEU3, regulates integrin-mediated cell migration via changes in glycolipid compositionDownload
The human neuraminidase enzyme, NEU3, is known to cleave sialic acid from ganglioside substrates. However, the biological effects of this cleavage are not clearly understood. This thesis describes our studies of the regulation of β1-integrins by neuraminidase enzymes, and the role of gangliosides...