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Fall 2020

Lu, Xiaocen

describe my efforts in development of the second generation of PhoCl variants (PhoCl2) with improved rate and efficiency of dissociation. To better understand the structural changes associated with photoconversion and peptide dissociation, we determined the X-ray crystal structures of the green state, the

red state, and the cleaved empty barrel of PhoCl1. Guided by the structural information, we further engineered PhoCl using a NanoLuc luciferase-based complementation assay for screening. Two variants of the second generation of PhoCl variants were developed via directed evolution. These variants were

nanoparticles by engineering protein hybrids composed of encapsulin and a light-sensitive protein. Encapsulin is a virus capsid-like natural nanocapsule with diameter of 25 nm to 32 nm, which is used by bacteria to isolate toxic enzymatic activities. I demonstrated in mammalian cell expression system that

Fall 2018

Astleitner, Katharina

This thesis studies integrative augmentation with customer satisfaction (CS) standards in a service and a manufacturing organization. Case study organization 1 (CSO 1) is an engineering course operated in a Western Canadian university. In this course, surveys were undertaken that addressed student

structural equation modelling (SEM) that illustrates the effects of multiple characteristics of the MS elements on e-class site satisfaction. The second analysis presents the results of an augmentative integration performed in a windows and doors manufacturer (CSO 2). The integrated management system (IMS

Fall 2022

McCutcheon, Jaclyn G

-sulfamethoxazole, whereas AXL3 is a novel virulent phage and candidate for genetic engineering. Additionally, I investigated the lack of in vivo data for S. maltophilia and show that type IV pili-binding phage DLP3 rescues Galleria mellonella larvae from lethal S. maltophilia infection. Further investigation into

the mechanism of host interactions for the type IV pili binding phages identified the surface exposed αβ-loop of the major pilin protein as a structural region important for phage binding, as well as two tail proteins in phage DLP2 as putative receptor binding proteins for cross-genera bacterial

Fall 2014

Beaudry, Allan L

In this thesis, a new technique named vapour-liquid-solid glancing angle deposition (VLS-GLAD) will be used to enhance structural control over branched nanowire (NW) arrays. NWs are 1D crystals that have been extensively applied in sensors, photovoltaic devices, and transistors. The functional

also be used to enable the fabrication of azimuthally aligned nanotrees without requiring epitaxy at the substrate. Aperiodic signals will be encoded into diameter oscillations along the length of branches by engineering their local shadowing environment using dynamic substrate motion during growth

Spring 2013

Davis, Elisabeth M

differentially with metal surfaces, suggesting that metal surfaces have chiral structural elements that are differentially recognized by the peptides. The addition of a PEG molecule to the RBD generates a facile, single-step versatile surface coating that increases the hardness of metals (304 stainless steel

industry for the facile generation of versatile surface engineering approaches for metals using non-toxic materials. The novel nanowire and environmental sensor aspects of T4P function have important implications in the pathogenesis of P. aeruginosa and further demonstrate the complexity and

Fall 2023

Li, Hangxuan

transition metal-based catalysts are demonstrated. Effective strategies such as alloying design, single-atom catalyst design, interface engineering, and heterojunction construction are summarized. Chapter 2 clarifies the objectives and experimental design based on the literature review. Chapter 3 is an

experimental section for the design and fabrication of transition metal-based heterogeneous structural Co2P-Ni3S2 hollow nanowires supported on nickel foam (Co2P-Ni3S2/NF). Owing to the multiple active sites provided by transition metal compounds, the large surface area of the unique hollow nanowire morphology

Fall 2023

Brassard, Leah

skills create cascades of resilience. Despite the CRM’s strong theoretical underpinnings, it has yet to be empirically tested with a community sample. Therefore, the present study tests the CRM’s proposed pathways for how parenting qualities can lead to family resilience. Self-report data from 295 self

-identified parents was collected on parental self-efficacy, positive emotions, coping capacity, social supports, and family resilience. Structural equation modelling was used to test the CRM, and showed that the proposed pathways of the CRM did not fully explain the data collected. However, adding a direct

Spring 2023

Grewal, Charnpal

complexes are stable, soluble, and monodisperse. This was followed by EMSAs (electrophoretic mobility shift assays) revealing that broadly, SF1 has a major role in binding the 3′ SS and modulates sequence specificity and affinity of the U2AF/SF1 complex for the 3′ SS. Structural characterization via SAXS

SF1 during the splicing cycle, therefore these structures contribute to a unified model of 3′ SS recognition. Ultimately, this thesis is important foundational work for understanding 3′ SS recognition since it is the first published report of the successful expression and purification of the

Spring 2020

Chu, Chun-Jui

common structural motif. LOS-I is a pentasaccharide that contains a trehalose core with three hydroxyl groups acylated with fatty acids. In LOS-II and LOS-III, this pentasacccharide is modified by the addition of one or two residues of a rare branched monosaccharide, caryophyllose. LOS-IV consists of LOS

future. Finally, I will report the synthesis of a protected derivative of LOS-I, starting from the triacylated trehalose core, via its coupling with different glycosyl fluorides to give the pentasaccharide backbone. Unfortunately, the final deprotection was unsuccessful and thus the synthesis of LOS-I

Fall 2016

Jeon, Jaechun

controlled by the strength of the magnetic field. By contrast, such resistance jumps are virtually non-existent in the 25 um by 25um microbridge. The results are described within a model of percolation or de-percolation of metallic domains within the confined region of the thin film. We also report the

creation of colossal in-plane anisotropic magnetoresistance (CAMR) of > 16,000 % via the engineering of spatial confinement in LPCMO films. Recalling that typical AMR values in films are only a few percent, these results mark an astonishing increase which could shed new light on the nature of the AMR in

, and can also provide important clues on engineering next generation EPS-based applications such as low field magnetoresistance sensor (LF-MR sensors), C-AMR hard disk read head, and magnetoresistive random access memory (M-RAM) devices.