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Fall 2024
MicroRNA is a class of endogenous, noncoding, short RNA molecules and the aberrant expression of microRNA is related to the development of a variety of human diseases. The detection of microRNA in living cells is vital to understanding its dynamic functions and profiles, diagnosing diseases, and...
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Understanding the trans-cleavage Activity of CRISPR-Cas Systems for RNA Detection Applications
DownloadFall 2022
The discovery and characterization of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems with trans-cleavage activity led to the rise of CRISPR-based diagnostics. A ribonucleoprotein (RNP), consisting of a CRISPR-associated protein (Cas) and a CRISPR RNA (crRNA) or single...