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Spring 2016

Varadarajan, Ramya

maintaining pre-meiotic oocyte arrest in many animal models. Earlier reports from the Campbell lab, however, have shown that loss of Myt1 activity affected multiple aspects of Drosophila spermatogenesis resulting in male sterility. The conserved meiotic checkpoint function of Myt1 was hypothesized to account

overall coordination of G2/MI transition. Instead, the phenotypic analysis of myt1 mutants indicated that Myt1 activity is required for structural integrity of a germline specific membranous cytoskeletal organelle called the fusome (or intercellular bridges). I found that inhibition of Cyclin A-Cdk1

Fall 2022

McCutcheon, Jaclyn G

-sulfamethoxazole, whereas AXL3 is a novel virulent phage and candidate for genetic engineering. Additionally, I investigated the lack of in vivo data for S. maltophilia and show that type IV pili-binding phage DLP3 rescues Galleria mellonella larvae from lethal S. maltophilia infection. Further investigation into

the mechanism of host interactions for the type IV pili binding phages identified the surface exposed αβ-loop of the major pilin protein as a structural region important for phage binding, as well as two tail proteins in phage DLP2 as putative receptor binding proteins for cross-genera bacterial

Fall 2024

Cho, Timothy HS

orientation may be present in other sensor kinases, revealing a previously unknown diversity in the structure of these common sensory domains. In Chapters 3 and 4, we investigate how the outer membrane lipoprotein NlpE activates CpxA in the presence of diverse inducing signals. We report the molecular details

of the interaction between NlpE and CpxA when NlpE is mislocalized to the inner membrane and examine the structural features of NlpE and their contribution to activating CpxA. We also find a role for the Cpx-regulated proteolytic factors CpxP and DegP in stabilizing NlpE, a novel axis of regulating

signaling from the outer membrane is coordinated through the cell wall. Finally, we report that NlpE can become surface-exposed, which may explain its ability to sense adhesion to surfaces. Thus, NlpE’s versatility in signaling comes from its ability to localize to both the inner and outer membranes, the

Fall 2020

Dueck, Meghan

accompanied by morphological novelties, structural reductions and losses, hyperossifications, and increased intraspecific variation, which can create difficulties when establishing natural classifications. Traditionally, all “small” species of Alestidae with reduced multicuspid teeth were grouped in the tribe

size series for P. conserialis that addresses previous discrepancies in the literature regarding the presence or absence of a parietal fontanelle and reports the presence of a parietal fontanelle in all examined specimens. I document negative allometric growth between standard length and the length and

Fall 2015

Dwivedi, Ritika

important to understand their generation, abundance and specific role(s). In addition, the mechanism of protein N-glycosylation and fOS generation by PglB are not well understood. This PhD thesis focuses on developing tools to study the generation, abundance and structural diversity of fOS. In addition, new

different between the two strains as determined by Western blot analysis with anti-N-glycan antibodies. This is the first report of the N-glycosylation of an OTase enzyme possibly affecting its own enzymatic activities. In order to better understand the N-glycosylation activity of PglB, a fluorescence

Fall 2019

Zwozdesky, Myron A

structural and phylogenetic relationships with vertebrate IgSF receptor-types. This family contains stimulatory and inhibitory forms that regulate several innate immune cell effector responses via classical as well as novel cytoplasmic tail (CYT)-dependent signaling capabilities. My thesis is focused on

demonstrated novel inhibitory signaling capabilities for IpLITR 1.1b, including the first report of cross-talk signaling potential within a teleost immunoregulatory receptor family. My work shows that teleost immunoregulatory receptors are excellent models to advance the understanding of innate