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- 2Prions
- 2Protein misfolding cyclic amplification
- 1Amplification
- 1Blood
- 1Chronic wasting disease
- 1De novo
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Characterizing patterns of tissue tropism and environmental reservoirs of PrPCWD in infected cervids
DownloadFall 2013
Chronic wasting disease (CWD) is a fatal prion disease affecting cervids. Believed to be transmitted horizontally, the factors affecting CWD contagion are unknown, but bodily fluids or excretions have been implicated as possible routes of transmission. Protein misfolding cyclic amplification...
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Optimization, adaptation and application of protein misfolding cyclic amplification to detection of prions in blood plasma
DownloadFall 2010
The PMCA assay was optimized for adaptation to low level detection of PrPSc in hamster plasma. Evaluation of numerous key variables of the PMCA assay led to an optimized protocol capable of ~3 log10 amplification after 32 cycles (two 16 hour rounds). When commercially purchased normal hamster...
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