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Fall 2020
Background: The CRISPR/Cas9 system has emerged as a revolutionary genetic engineering technology capable of editing various cell types, creating disease models, and more recently, changing human DNA1 . It consists of three pieces: the Cas9 endonuclease, the gRNA, and the DNA target. In short, the...
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Fall 2022
The ability to reliably target specific sequences of interest is a critical component of any gene editing technology. While the discovery of CRISPR-Cas systems have fundamentally simplified the way in which genomic material may be manipulated, the presence of off-target activity at undesired loci...