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Skip to Search Results- 1Agricultural, Food and Nutritional Science, Department of
- 1Agricultural, Food and Nutritional Science, Department of/Journal Articles (Agricultural, Food and Nutritional Science)
- 1Graduate and Postdoctoral Studies (GPS), Faculty of
- 1Graduate and Postdoctoral Studies (GPS), Faculty of/Theses and Dissertations
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Comparative assessment of qPCR enumeration methods that discriminate between live and dead Escherichia coli O157:H7 on beef
Download2018-01-01
Laidlaw, Anna M., Gänzle, Michael G., Yang, Xianqin
Quantitative Polymerase Chain Reaction (qPCR) is a molecular method commonly used to detect and quantify bacterial DNA on food but is limited by its inability to distinguish between live and dead cell DNA. To overcome this obstacle, propidium monoazide (PMA) alone or with deoxycholate (DC) was...
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Development and use of a gnotobiotic murine model for beef cattle to evaluate competitive exclusion of Escherichia coli O157:H7 using commensal Escherichia coli strains, and to ascertain the impact of physiological stress on the host-bacteria interaction
DownloadFall 2020
Enterohemorrhagic Escherichia coli (EHEC) serotype O157:H7 is an important foodborne pathogen, and cattle are considered the primary reservoir of this bacterium. Research was undertaken to ascertain factors that regulate competitive exclusion of E. coli O157:H7. A gnotobiotic (GB) murine model...