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Skip to Search Results- 1Attia, Karen
- 1Cromwell, Christopher Robert
- 1Feng, Wei
- 1Friesen, Eric R.
- 1Gesner, Emily
- 1Huynh, Nhan
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Characterizing new players involved in iron homeostasis during Drosophila larval development: Shifting the classic paradigm of iron metabolism
DownloadFall 2020
Iron plays a critical role in many biological processes. The ability of iron to gain and lose electrons allows it to be involved in a wide variety of biochemical reactions. Organismal iron is commonly found in two types of protein cofactors: heme and iron-sulfur clusters (ISCs). Iron is critical...
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Fall 2021
Background: Clustered Regularly Interspaced Palindromic Repeats, or CRISPR, is an array of DNA sequences found in bacteria that confer immunity against phage. Sequences within the CRISPR array are transcribed into CRISPR RNA (crRNA), which hybridize with CRISPR associated proteins (Cas) to search...
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Fall 2022
Hepatitis B virus (HBV) has infected two billion people worldwide, culminating in approximately 250 million chronic carriers. Chronic HBV carriers are at high risk of developing severe liver diseases, such as cirrhosis and cancer, resulting in an estimated 600,000 HBV-associated deaths annually....
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Fall 2022
The precise and targeted modification of cell genomes has been an important goal since the discovery of the relationship between DNA sequence and the phenotypes of organism. Many tools have emerged to address this challenge, the most important one being the CRISPR-Cas9 system. This technology...
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Fall 2023
Phosphoinositides (PIs) are membrane lipids that function as signaling molecules. PIs undergo phosphorylation and dephosphorylation at various positions to produce the seven known PI molecules. PIKfyve is a phosphoinositide kinase that produces phosphoinositide-3,5- bisphosphate (PI(3,5)P2) and...
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Stable knock-in of duck RIG-I into DF-1 chicken cells via CRISPR/Cas9 homology directed repair
DownloadFall 2020
Chickens and ducks differ in their susceptibility to avian influenza virus infection. Ducks are the primordial hosts and reservoir of avian influenza A viruses. Mallard ducks can asymptomatically harbour high-pathogenicity avian influenza (HPAI) and low-pathogenicity avian influenza (LPAI)....
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Spring 2011
Powerful mechanisms of genetic interference in both unicellular and multicellular organisms are based on the sequence-directed targeting of DNA or RNA by small effector RNAs. In many bacteria and almost all archaea, RNAs derived from clustered, regularly interspaced, short palindromic repeat...
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Fall 2022
The ability to reliably target specific sequences of interest is a critical component of any gene editing technology. While the discovery of CRISPR-Cas systems have fundamentally simplified the way in which genomic material may be manipulated, the presence of off-target activity at undesired loci...
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Understanding the trans-cleavage Activity of CRISPR-Cas Systems for RNA Detection Applications
DownloadFall 2022
The discovery and characterization of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) systems with trans-cleavage activity led to the rise of CRISPR-based diagnostics. A ribonucleoprotein (RNP), consisting of a CRISPR-associated protein (Cas) and a CRISPR RNA (crRNA) or single...