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Skip to Search Results- 9Fluorescent proteins
- 8Biosensors
- 4Protein engineering
- 2Directed evolution
- 1Bimolecular fluorescence complementation
- 1Calcium
- 1Abdelfattah, Ahmed
- 1Alford, Spencer Caleb
- 1Assempour, Nazanin
- 1Belal, Ahmed S. F.
- 1Carlson, Haley J
- 1Hoi, Hiofan
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Carbon-on-Gold Films and Spontaneous Adsorption of Aryldiazonium Salts for Surface Plasmon Resonance Sensing
DownloadFall 2021
Over the past decades, surface plasmon resonance (SPR) technology has gained popularity as a powerful analytical technique for the study of biomolecular interactions and medical diagnostics. This is made possible thanks to the well-established gold-thiolate chemistry. However, the limited...
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Spring 2013
The discovery of green fluorescent protein (GFP) from the Aequorea victoria jellyfish revolutionized many fields in the scientific community, including molecular biology, protein engineering, and neuroscience. The ability to genetically link a fluorescent protein to a protein of interest has...
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Fall 2016
Fluorescence microscopy is currently the most powerful imaging technique for interrogation of neural circuits. Accordingly, tremendous efforts have been invested in engineering fluorescent proteins (FPs) to act as indicators for various biochemical processes in neural circuits, resulting in a...
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Fall 2012
Fluorescent proteins (FPs) are indispensible biochemical tools. The concerted efforts of protein engineers have produced FPs spanning the visible colour spectrum. This wide variety of FPs has greatly facilitated the development of FP-based biosensors. However, researchers rely on relatively few...
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Spring 2013
Fluorescent protein (FP) technology is now an indispensible tool of biomedical research. Nevertheless, only a few members of the hundreds of existing FPs are generally regarded as the preferred options for most imaging applications. Accordingly, new FPs with ever-improved properties are in demand...
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Fall 2013
The discovery of green fluorescent protein (GFP) from the Aequorea victoria Jellyfish followed by the extensive efforts of protein engineers to produce other fluorescent proteins (FPs) spanning the visible color spectrum made fluorescent proteins indispensable biochemical tools in the scientific...
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Fall 2014
The discovery and subsequent applications of fluorescent proteins (FPs) launched a new era for live cell fluorescence imaging. The design and developments of FP-based indicators have further solidified the versatility of FPs and rendered them as indispensable tools in life science research now...
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Directed Evolution Approaches for Improved Genetically Encoded Fluorescent Calcium Ion and Voltage Indicators
DownloadFall 2014
Fluorescent proteins (FP) have revolutionized our imaging technologies and facilitated visualization of biochemical and physiological processes occurring in complex biological systems, which has opened up a new and unprecedented era in cell biology. Although FPs mainly serve as passive...
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Spring 2015
Fluorescent proteins (FP) and FP-based biosensors have become essential tools for cell biology and neuroscience research. This thesis describes efforts to engineer new FPs with red emission and novel genetically encoded biosensors based on red FPs. Directed evolution and semi-rational design are...
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Fall 2015
The expansion of the fluorescent protein (FP) colour palette through discovery of more species possessing FPs and by subjecting them to intensive protein engineering efforts has greatly aided the effectiveness of FP-based biosensor designs. Currently, the main FP-based methods for detecting...