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2004

Zhang, Chen-Yu, Chan, Catherine B., Koshkin, Vasilij, Wheeler, Michael B., Lowell, Bradford B., Joseph, Jamie W., Saleh, Monique C., Sivitz, William I.

Chronic exposure to elevated free fatty acids (lipotoxicity) induces uncoupling protein (UCP2) in the pancreatic β-cell, and therefore a causal link between UCP2 and β-cell defects associated with obesity may exist. Recently, we showed that lipid treatment in vivo and in vitro in UCP2(–/–)...

2005

Chan, Catherine B., Wheeler, Michael B., Asghar, Zeenat, Diao, Jingyu

The insulin receptor (IR) and its signaling appear to be essential for insulin secretion from pancreatic β-cells. However, much less is known about the role of the IR in α-cells. To assess the role of the IR in glucagon and insulin secretion, we engineered adeno-viruses for high efficiency small...

2001

Arumugam, Yoga, Luiken, Joost J. F. P., Dyck, David J., Bonen, Arend, Tandon, Narendra N., Pelsers, Maurice M. L., Bell, Rhonda C., Turcotte, Lorraine P., Glatz, Jan F. C.

Giant vesicles were used to study the rates of uptake of long-chain fatty acids by heart, skeletal muscle, and adipose tissue of obese and lean Zucker rats. With obesity there was an increase in vesicular fatty acid uptake of 1.8-fold in heart, muscle and adipose tissue. In some tissues only...

2008

Wheeler, Michael B., Chan, Catherine B., Dai, Feihan F., Koshkin, Vasilij, Robson-Doucette, Christine A.

Involvement of the mitochondrial permeability transition (MPT) pore in early stages of lipotoxic stress in the pancreatic β-cell lines MIN6 and INS-1 was the focus of this study. Both long term (indirect) and acute (direct) effects of fatty acid (FA) application on β-cell susceptibility to...

2003

Chan, Catherine B., Wang, Xiaolin, Koshkin, Vasilij, Wheeler, Michael B., Scherer, Philipp E.

Excessive free fatty acid (FFA) exposure represents a potentially important diabetogenic condition that can impair insulin secretion from pancreatic β-cells. Because mitochondrial oxidative phosphorylation is a main link between glucose metabolism and insulin secretion, in the present work we...

2004

Chan, Catherine B., Bikopoulos, George, Koshkin, Vasilij, Wheeler, Michael B.

Mitochondrial permeability transition (MPT), which contributes substantially to the regulation of normal mitochondrial metabolism, also plays a crucial role in the initiation of cell death. It is known that MPT is regulated in a tissue-specific manner. The importance of MPT in the pancreatic...