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Skip to Search Results- 6Protein–ligand complexes
- 4Hydrophobic
- 2ADPKD
- 2Collision-induced dissociation
- 2Dissociation kinetics
- 2Electrospray ionization mass spectrometry
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Dissociation kinetics of the streptavidin-biotin interaction measured using direct electrospray ionization mass spectrometry analysis
Download2013
Kitova, Elena N., Klassen, John S., Deng, Lu
Dissociation rate constants (koff) for the model high affinity interaction between biotin (B) and the homotetramer of natural core streptavidin (S4) were measured at pH 7 and temperatures ranging from 15 to 45 °C using electrospray ionization mass spectrometry (ESI-MS). Two different approaches...
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Dissociation kinetics of the streptavidin-biotin interaction measured using direct electrospray ionization mass spectrometry analysis
Download2013
Deng, Lu, Klassen, John S., Kitova, Elena N.
Dissociation rate constants (koff) for the model high affinity interaction between biotin (B) and the homotetramer of natural core streptavidin (S4) were measured at pH 7 and temperatures ranging from 15 to 45 °C using electrospray ionization mass spectrometry (ESI-MS). Two different approaches...
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2014
Kitova, Elena N., Klassen, John S., Michelsen, Klaus, Liu, Lan, Schnier, Paul D., Baergen, Alyson
This work explores the energetics of intermolecular H-bonds inside a hydrophobic protein cavity. Kinetic measurements were performed on the gaseous deprotonated ions (at the −7 charge state) of complexes of bovine β-lactoglobulin (Lg) and three monohydroxylated analogs of palmitic acid (PA):...
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2014
Kitova, Elena N., Klassen, John S., Michelsen, Klaus, Liu, Lan, Schnier, Paul D., Baergen, Alyson
This work explores the energetics of intermolecular H-bonds inside a hydrophobic protein cavity. Kinetic measurements were performed on the gaseous deprotonated ions (at the −7 charge state) of complexes of bovine β-lactoglobulin (Lg) and three monohydroxylated analogs of palmitic acid (PA):...
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Spring 2013
Autosomal dominant polycystic kidney disease (ADPKD) is an inherited genetic disorder, inducing cysts in kidney, liver and pancreas. It is caused by pathogenic mutations in the PKD1 or PKD2 gene encoding PKD1 or PKD2 respectively. Cellular abnormalities in ADPKD include cell over-proliferation...
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Function and Regulation of TRPP2 and TRPP3, and identification of pore gates of TRP channels
DownloadSpring 2017
The transient receptor potential (TRP) superfamily of cation channels is composed of eight subfamilies, TRPC/V/M/P/ML/A/N/Y, and play distinct sensory roles in response to various environmental stimuli. TRPP2, or polycystin-2, is mutated in autosomal dominant polycystic kidney disease (ADPKD)....
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Mapping protein-ligand interactions in the gas phase using a functional group replacement strategy: Comparison of CID and BIRD activation methods
Download2013
Klassen, John S., Deng, Lu, Kitova, Elena N.
Intermolecular interactions in the gaseous ions of two protein–ligand complexes, a single chain antibody (scFv) and its trisaccharide ligand (α-D-Galp-(1→2)-[α-D-Abep-(1→3)]-α-Manp-OCH3, L1) and streptavidin homotetramer (S4) and biotin (B), were investigated using a collision-induced...
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Mapping protein-ligand interactions in the gas phase using a functional group replacement strategy: Comparison of CID and BIRD activation methods
Download2013
Klassen, John S., Kitova, Elena N., Deng, Lu
Intermolecular interactions in the gaseous ions of two protein–ligand complexes, a single chain antibody (scFv) and its trisaccharide ligand (α-D-Galp-(1→2)-[α-D-Abep-(1→3)]-α-Manp-OCH3, L1) and streptavidin homotetramer (S4) and biotin (B), were investigated using a collision-induced...
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Fall 2013
Polymers are widely used as coatings, adhesives, biomaterials and many other applications. These successful applications rely on the physical and chemical properties of the polymer molecules and surfaces. One type of important molecular and surface interaction is hydrophobic interaction which is...