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Probing microscopic conformational dynamics in folding reactions by measuring transition paths
Download2019-12-01
Hoffer, Noel Q, Woodside, Michael T
Transition paths comprise those parts of a folding trajectory where the molecule passes through the high-energy transition states separating folded and unfolded conformations. The transition states determine the folding kinetics and mechanism but are difficult to observe because of their brief...
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2021-10-01
Petrosyan, Rafayel, Narayan, Abhishek, Woodside, Michael T
The use of force probes to induce unfolding and refolding of single molecules through the application of mechanical tension, known as single-molecule force spectroscopy (SMFS), has proven to be a powerful tool for studying the dynamics of protein folding. Here we provide an overview of what has...