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- 3Liquid sample DESI-MS
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Comparison of the Responsivity of Solution-Suspended and Surface-Bound Poly (N-isopropylacrylamide)-Based Microgels for Sensing Applications
Download2017-01-01
Li, Wenxiang, Hu, Liang, Zhu, Jinghua, Li, Dan, Luan, Yafei, Xu, Wenwen, Serpe, Michael J.
In this submission, the phase transition behavior for poly(N-isopropylacrylamide-co-acrylic acid) (pNIPAm-co-AAc) microgels and their assemblies was investigated as a function of temperature and pH using UV–vis spectroscopy (to probe light scattering behavior) and quartz crystal microbalance with...
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Development and Application of ESI-MS Based Techniques for Quantitative Determination of Protein-Carbohydrate Interactions
DownloadFall 2016
This thesis describes the development and application of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was employed to quantify protein-carbohydrate interactions in aqueous buffer...
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Fall 2016
This thesis describes the development of mass spectrometry methods to study protein-ligand interactions in vitro. Liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) was first applied to quantify protein-carbohydrate interactions in aqueous ammonium acetate...
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Electrically Triggered Small Molecule Release from Poly (N-Isopropylacrylamide-co-Acrylic Acid) Microgel-Modified Electrodes
Download2018-01-01
Xu, Wenwen, Zhang, Y., Gao, Yongfeng, Serpe, Michael J.
A monolithic layer of poly(N-isopropylacrylamide-co-acrylic acid) microgels was deposited on an Au electrode and used for electrically triggered release of the small molecule crystal violet (CV), which was used as a model drug. CV was loaded into the surface-bound microgels by exposing them to a...
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2014
Serpe, Michael J., Xu, Wenwen, Gao, Yongfeng
In this submission, pH responsive poly(N-isopropylacrylamide)–based microgels were used to fabricate optical devices (etalons). We demonstrate that the optical properties of the etalons could be manipulated upon the application of an appropriate electric potential between the etalon and a counter...
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2014
Serpe, Michael J., Xu, Wenwen, Gao, Yongfeng
Poly (N-isopropylacrylamide-co-acrylic acid) (pNIPAm-co-AAc) microgel-based free-standing etalons have been developed by fabricating etalons on a solid support that has been coated with a sacrificial polymer layer. Therefore, the etalon could be easily removed from the solid substrate upon...
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2016
Klassen, John S., Yao, Yuyu, Kitova, Elena N., Richards, Michele R.
The results of an investigation into the influence of sulfolane, a commonly used supercharging agent, on electrospray ionization mass spectrometry (ESI-MS) measurements of protein–ligand affinities are described. Binding measurements carried out on four protein–carbohydrate complexes, lysozyme...
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2016
Klassen, John S., Yao, Yuyu, Kitova, Elena N., Richards, Michele R.
The results of an investigation into the influence of sulfolane, a commonly used supercharging agent, on electrospray ionization mass spectrometry (ESI-MS) measurements of protein–ligand affinities are described. Binding measurements carried out on four protein–carbohydrate complexes, lysozyme...
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2017-01-01
Xu, Wenwen, Wei, Menglian, Serpe, Michael J.
A facile self‐assembly method is presented to prepare poly(N‐isopropylacrylamide)‐based microgels modified anisotropically with gold nanoparticles (Au NPs) to yield Janus microgels. Transmission electron microscopy is used to confirm that microgels are selectively coated on one or both sides with...
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2015
Klassen, John S., Yao, Yuyu, Serpe, Michael J., Xu, Wenwen
Anisotropic particles have generated an enormous amount of research interest due to their applications for drug delivery, electronic displays and as micromotors. However, up till now, there is no single protocol capable of generating particles of “patchy” composition with a variety of...