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2016
Richards, Michele R., Campuzano, Iain D. G., Kitova, Elena N., Klassen, John S., Li, Jun, Privé, Gilbert G., Xiong, Zi Jian, Bagal, Dhanashri
Saposin A (SapA) lipoprotein discs, also known as picodiscs (PDs), represent an attractive method to solubilize glycolipids for protein interaction studies in aqueous solution. Recent electrospray ionization mass spectrometry (ESI-MS) data suggest that the size and composition of...
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2016
Klassen, John S., Campuzano, Iain D. G., Bagal, Dhanashri, Privé, Gilbert G., Kitova, Elena N., Xiong, Zi Jian, Richards, Michele R., Li, Jun
Saposin A (SapA) lipoprotein discs, also known as picodiscs (PDs), represent an attractive method to solubilize glycolipids for protein interaction studies in aqueous solution. Recent electrospray ionization mass spectrometry (ESI-MS) data suggest that the size and composition of...
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2014
Serpe, Michael J., Xu, Wenwen, Gao, Yongfeng
In this submission, pH responsive poly(N-isopropylacrylamide)–based microgels were used to fabricate optical devices (etalons). We demonstrate that the optical properties of the etalons could be manipulated upon the application of an appropriate electric potential between the etalon and a counter...
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2014
Serpe, Michael J., Xu, Wenwen, Gao, Yongfeng
Poly (N-isopropylacrylamide-co-acrylic acid) (pNIPAm-co-AAc) microgel-based free-standing etalons have been developed by fabricating etalons on a solid support that has been coated with a sacrificial polymer layer. Therefore, the etalon could be easily removed from the solid substrate upon...
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2016
Klassen, John S., Yao, Yuyu, Kitova, Elena N., Richards, Michele R.
The results of an investigation into the influence of sulfolane, a commonly used supercharging agent, on electrospray ionization mass spectrometry (ESI-MS) measurements of protein–ligand affinities are described. Binding measurements carried out on four protein–carbohydrate complexes, lysozyme...
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2016
Klassen, John S., Yao, Yuyu, Kitova, Elena N., Richards, Michele R.
The results of an investigation into the influence of sulfolane, a commonly used supercharging agent, on electrospray ionization mass spectrometry (ESI-MS) measurements of protein–ligand affinities are described. Binding measurements carried out on four protein–carbohydrate complexes, lysozyme...
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2015
Klassen, John S., Yao, Yuyu, Serpe, Michael J., Xu, Wenwen
Anisotropic particles have generated an enormous amount of research interest due to their applications for drug delivery, electronic displays and as micromotors. However, up till now, there is no single protocol capable of generating particles of “patchy” composition with a variety of...
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Protecting group-free immobilization of glycans for affinity chromatography using glycosylsulfonohydrazide donors
Download2015-09-16
Heranadez Armada, Daniel, Santos, Jobette T., Richards, Michele R., Cairo, Christopher W.
A variety of applications in glycobiology exploit affinity chromatography through the immobilization of glycans to a solid support. Although several strategies are known, they may provide certain advantages or disadvantages in how the sugar is attached to the affinity matrix. Additionally, the...
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Quantifying protein-carbohydrate interactions using liquid sample desorption electrospray ionization mass spectrometry
Download2015
N. Kitova, Elena N., Shams-Ud-Doha, Km, Yao, Yuyu, Klassen, John S., Daneshfar, Rambod
The application of liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) for quantifying protein–carbohydrate interactions in vitro is described. Association constants for the interactions between lysozyme and β-D-GlcNAc-(1 → 4)-β-D-GlcNAc-(1 → 4)-D-GlcNAc and...
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Quantifying protein-carbohydrate interactions using liquid sample desorption electrospray ionization mass spectrometry
Download2015
Klassen, John S., N. Kitova, Elena N., Yao, Yuyu, Shams-Ud-Doha, Km, Daneshfar, Rambod
The application of liquid sample desorption electrospray ionization mass spectrometry (liquid sample DESI-MS) for quantifying protein–carbohydrate interactions in vitro is described. Association constants for the interactions between lysozyme and β-D-GlcNAc-(1 → 4)-β-D-GlcNAc-(1 → 4)-D-GlcNAc and...